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Comparative transcriptome characterization of paired primary gastric cancer and ovarian metastasis

ABSTRACT: Comparative transcriptome characterization of paired primary gastric cancer and ovarian metastasis

PROVIDER: PRJNA789867 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17262719_1.fastq.gz	Fastqsanger.gz
	SRR17262719_2.fastq.gz	Fastqsanger.gz
	SRR17262720_1.fastq.gz	Fastqsanger.gz
	SRR17262720_2.fastq.gz	Fastqsanger.gz
	SRR17262721_1.fastq.gz	Fastqsanger.gz

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Comparative transcriptome characterization of paired primary gastric cancer and ovarian metastasis

Project description:To understand the molecular mechanism underlying ovarian metastasis of gastric cancer, we performed RNA-seq of paired primary tumor, normal mucosa and ovarian metastasis of four GC patients by the Illumina sequencing platform with 150-bp paired-end, followed by functional enrichment analyses of differentially expressed genes between three sample sets. A total number of 15,493 protein-coding genes were detected, the majority of which were the annotated human reference genes. Using a threshold of fold change >2 and adjusted P value <0.05, a total number of 3023 differentially expressed genes were detected between different sets of samples. Among them, 479 and 609 protein-coding genes were up- and down-regulated in ovarian metastases over primary tumors, respectively. Functional enrichment analyses revealed the significant enrichment of immune system, tumor microenvironment, metabolism and sex hormone-related pathways in the ovarian metastasis of gastric cancer. In conclusion, comparative transcriptome characterization of paired primary and ovarian metastatic tumor profiled the genome-wide molecular expression and unveiled functionally enriched pathways underlying this specific type of distant metastasis in GC.

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