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Gene expression in murine leukemic B cells treated with leukemic extracellular vesicles

ABSTRACT: Gene expression in murine leukemic B cells treated with leukemic extracellular vesicles

PROVIDER: PRJNA789946 | ENA |

REPOSITORIES: ENA

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Gene expression in murine leukemic B cells treated with leukemic extracellular vesicles

Project description:mRNA profiles of murine leukemic B cells (Eu-TCL1 mice) treated with extracellular vesicles were generated by 3'-sequencing, in triplicate.

2022-08-31 | GSE191186 | GEO

Gene expression in murine CD8+ T cells treated with leukemia extracellular vesicles

Project description:Gene expression in murine CD8+ T cells treated with leukemia extracellular vesicles

| PRJNA788480 | ENA

Transcriptomic analysis of human regulatory T cells treated with chronic myeloid leukemia-derived extracellular vesicles

Project description:The aim of our study was to evaluate if and how leukemic extracellular vesicles (EVs) modulate human regulatory T cells (Treg). Treg in myeloid leukemias have a pro-leukemic function, but mechanisms driving these cells are unknown. We hypothesised that leukemia-derived extracellular vesicles (EVs) may be a factor that polarizes Treg into pro-leukemic cells. Using bulk RNA sequencing we study transcriptomic differences in human regulatory T cells (3 biological replicates = Treg from 3 different donors) treated with leukemic EVs released by chronic myeloid leukemia K562 cells. We observed significant transcriptomic differences, e.g. in genes responsible for RNA processing and alternative splicing, as well as genes engaged in T cell maturation, origin and activity. Using obtained datasets we also identified transcription factors engaged in this regulation. RNA sequencing data is part of a bigger study, where we study leukemic EVs-Treg interaction using human regulatory T cells, analysis by multicolor flow cytometry, functional studies, in vivo models.

2022-03-30 | GSE180883 | GEO

Impact of Group Psychological Interventions on Extracellular Vesicles in People Who Had Cancer

Project description:Background: There is some evidence demonstrating the effect of psychological interventions in improvements in health biological parameters. To best of our knowledge, no study had addressed the impact of any psychological intervention on extracellular vesicles. In addition, Mindfulness-Based Cognitive Therapy (MBCT) and Emotion Focused Therapy for Cancer Recovery (EFT-CR) in the group have never been explored regarding extracellular vesicles and the effectiveness of these was not compared yet. Objectives: 1. To explore and compare the effect of MBCT and EFT-CR on biological parameters and psychological variables in distressed people who have had breast, prostate and colorectal cancer; 2. In addition, we will explore the acceptability through recruitment and retention rates of MBCT and EFT-CR in group and evaluate whether these interventions are appropriate for a larger clinical trial. Methods: The design of this study is a parallel randomized controlled trial. Participants will be randomized into MBCT, EFT-CR or usual care. Outcome measures will be assessed before, at the end of the intervention (8 weeks) and follow-ups (24 and 52 weeks from the baseline moment). Hypotheses: The researchers expected that both interventions will have an effect on extracellular vesicles and other study biomarkers as well as improvements in psychological outcomes, compared to treatment as usual (TAU) group. Regarding the comparative effectiveness, we did not have evidence to hypothesize which one of the interventions will be superior in both biological (extracellular vesicles) and psychological outcomes. Contribution for practice: The results of this preliminary study would permit to know if there are benefits of these psychological interventions on changes in extracellular vesicles and on psychological outcomes related to health. In addition, this study will permit to determine the acceptability of conducting a larger randomized controlled trial.

| 2332322 | ecrin-mdr-crc

Gene expression analysis in murine lymphocyte subsets incubated with control or leukemia-derived extracellular vesicles

Project description:mRNA profiles of Treg, CD4+, CD8+, CD19+ splenic cells from 8-week-old Foxp3 YFP/cre mice incubated with control (WT) or leukemic (TCL1) extracellular vesicles (EV) were analyzed in triplicate.

2022-08-31 | GSE188898 | GEO

RNA from RAW 264.7 cells treated with miR-loaded extracellular vesicles

Project description:RNA was harvested from RAW 264.7 cells treated with miR-loaded extracellular vesicles

2022-05-13 | GSE167752 | GEO

Extracellular vesicles

Project description:Extracellular vesicles Raw sequence reads

| PRJNA816091 | ENA

miRNA expression of extracellular vesicles

Project description:To further investigate the molecular mechanisms by which EVs mediated the abnormal localization of tight junction proteins and adherence junction protein, we performed miRNA microarray analysis of extracellular vesicles isolated from breast cancer cells. miRNA expression in extracellular vesicles was collected from MDA-MB-231-D3H1, MDA-MB-231-D3H2LN, BMD2a and BMD2b breast cancer cell lines.

2015-04-02 | E-GEOD-63445 | biostudies-arrayexpress

sRNAs from Neisseria extracellular vesicles

Project description:Similar to bacterial proteins that are targeted to distinct macrophages organelles via extracellular vesicles, we propose that these vesicles also traffic small RNAs to modulate specific host factors. To test this, we aim to sequence extracellular vesicle derived sRNA, and whole bacterial small RNAs to determine selectivity, and to identify their bacterial and mammalian targets (Experimental Plan in Table-1). For this we will collect highly purified vesicles from N. gonorrhoeae (strain MS11A). We will also treat mouse derived primary macrophages with extracellular vesicles and compare their RNA response to untreated macrophages (Table-2). This will provide novel insights into how macrophages respond to N. gonorrhoeae infections. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/

2016-03-01 | E-ERAD-439 | biostudies-arrayexpress

miRNA content in extracellular vesicles

Project description:comparison miRNA content between extracellular vesicles from AAV-transduced or intact cells

2021-08-07 | E-MTAB-10858 | biostudies-arrayexpress