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Gene expression in murine leukemic B cells treated with leukemic extracellular vesicles

ABSTRACT: Gene expression in murine leukemic B cells treated with leukemic extracellular vesicles

PROVIDER: PRJNA789946 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17267119.fastq.gz	Fastqsanger.gz
	SRR17267120.fastq.gz	Fastqsanger.gz
	SRR17267121.fastq.gz	Fastqsanger.gz
	SRR17267122.fastq.gz	Fastqsanger.gz
	SRR17267123.fastq.gz	Fastqsanger.gz

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Gene expression in murine leukemic B cells treated with leukemic extracellular vesicles

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Project description:The aim of our study was to evaluate if and how leukemic extracellular vesicles (EVs) modulate human regulatory T cells (Treg). Treg in myeloid leukemias have a pro-leukemic function, but mechanisms driving these cells are unknown. We hypothesised that leukemia-derived extracellular vesicles (EVs) may be a factor that polarizes Treg into pro-leukemic cells. Using bulk RNA sequencing we study transcriptomic differences in human regulatory T cells (3 biological replicates = Treg from 3 different donors) treated with leukemic EVs released by chronic myeloid leukemia K562 cells. We observed significant transcriptomic differences, e.g. in genes responsible for RNA processing and alternative splicing, as well as genes engaged in T cell maturation, origin and activity. Using obtained datasets we also identified transcription factors engaged in this regulation. RNA sequencing data is part of a bigger study, where we study leukemic EVs-Treg interaction using human regulatory T cells, analysis by multicolor flow cytometry, functional studies, in vivo models.

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Impact of Group Psychological Interventions on Extracellular Vesicles in People Who Had Cancer

Project description:Background: There is some evidence demonstrating the effect of psychological interventions in improvements in health biological parameters. To best of our knowledge, no study had addressed the impact of any psychological intervention on extracellular vesicles. In addition, Mindfulness-Based Cognitive Therapy (MBCT) and Emotion Focused Therapy for Cancer Recovery (EFT-CR) in the group have never been explored regarding extracellular vesicles and the effectiveness of these was not compared yet. Objectives: 1. To explore and compare the effect of MBCT and EFT-CR on biological parameters and psychological variables in distressed people who have had breast, prostate and colorectal cancer; 2. In addition, we will explore the acceptability through recruitment and retention rates of MBCT and EFT-CR in group and evaluate whether these interventions are appropriate for a larger clinical trial. Methods: The design of this study is a parallel randomized controlled trial. Participants will be randomized into MBCT, EFT-CR or usual care. Outcome measures will be assessed before, at the end of the intervention (8 weeks) and follow-ups (24 and 52 weeks from the baseline moment). Hypotheses: The researchers expected that both interventions will have an effect on extracellular vesicles and other study biomarkers as well as improvements in psychological outcomes, compared to treatment as usual (TAU) group. Regarding the comparative effectiveness, we did not have evidence to hypothesize which one of the interventions will be superior in both biological (extracellular vesicles) and psychological outcomes. Contribution for practice: The results of this preliminary study would permit to know if there are benefits of these psychological interventions on changes in extracellular vesicles and on psychological outcomes related to health. In addition, this study will permit to determine the acceptability of conducting a larger randomized controlled trial.

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