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Lactate treatment regulates transcriptome of mouse embryonic stem cells (mESCs)

ABSTRACT: Lactate treatment regulates transcriptome of mouse embryonic stem cells (mESCs)

PROVIDER: PRJNA790759 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17276439_1.fastq.gz	Fastqsanger.gz
	SRR17276439_2.fastq.gz	Fastqsanger.gz
	SRR17276440_1.fastq.gz	Fastqsanger.gz
	SRR17276440_2.fastq.gz	Fastqsanger.gz
	SRR17276441_1.fastq.gz	Fastqsanger.gz

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Lactate treatment regulates epigenome of mouse embryonic stem cells (mESCs)

Project description:Lactate treatment regulates epigenome of mouse embryonic stem cells (mESCs)

| PRJNA791242 | ENA

Lactate treatment regulates transcriptome of mouse embryonic stem cells (mESCs)

Project description:We treated mESCs with 50mM lactate to examine its impact on mESC transcriptome. Interestingly, we found that lactate supplementation activated both germline genes and cleavage embryo genes, indicating that lactate supplementation expands transcriptional network in mouse ESCs.

2022-02-01 | GSE191252 | GEO

Lactate treatment regulates epigenome of mouse embryonic stem cells (mESCs)

Project description:We treated mESCs with 50mM lactate to examine its impact on mESC epigenome. Interestingly, we found that lactate supplementation stimulated H3K18 lactylation accumulation on a subset of genes, which in turn promoted transcriptional elongation. Our results indicate that lactate supplementation expands transcriptional network of mouse ESCs.

2022-02-01 | GSE192358 | GEO

Wild-type, Foxa2-/-, and Nkx2.2-/- mESCs at different time points of differentiation

Project description:We report gene-expression profiling of different mouse embryonic stem cells (mESCs) mutant cell lines differentiating to neuronal lineages upon treatment with Retinoic Acid (RA) and Smoothened agonist (SAG). We generated genome-wide expression profiles of embryonic stem cells and neural progenitors at different differentiating time-points for different mutant cell lines and WT control mESCs. We find NKX2.2 and FOXA2 are key components of an intrinsic timer in neural stem cells founded on a negative feedback network that regulates the duration of motor neuron neurogenesis.

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Lactate regulates cell cycle and proliferation by remodeling the anaphase promoting complex

Project description:Lactate is abundant in rapidly dividing cells, due to the requirement for elevated glucose catabolism to support proliferation. However, it is not known whether accumulated lactate affects the proliferative state. Here, we deploy a systematic approach to determine lactate-dependent regulation of proteins across the human proteome. From this data, we elucidate a mechanism of cell cycle regulation whereby accumulated lactate activates the anaphase promoting complex (APC/C). Remodeling of APC/C in this way is caused by direct inhibition of the SUMO protease SENP1 by lactate. We discover that accumulated lactate binds and inhibits SENP1 by forming a complex with zinc in the SENP1 active site. SENP1 inhibition by lactate stabilizes SUMOylation of two residues on APC4, which are required to reorient APC/C to allow UBE2C binding and thus increase APC/C activity. This direct regulation of APC/C by lactate is required to stimulate timed degradation of cell cycle proteins, and efficient mitotic exit in proliferative mammalian cells. The above mechanism is initiated upon mitotic entry when lactate abundance reaches its apex. In this way, accumulation of lactate communicates the consequences of a nutrient replete growth phase to stimulate timed opening of APC/C, cell division, and proliferation. Conversely, we show that aberrant remodeling of APC/C by lactate occurs when lactate concentrations are chronically elevated, which is a hallmark of numerous cancers. Moreover, persistent lactate accumulation overcomes anti-mitotic pharmacology to drive mitotic slippage via the newfound mode of APC/C activation. Taken together, we define a biochemical mechanism through which lactate directly regulates protein function to control cell cycle and proliferation.

2023-02-07 | PXD036641 | Pride

RBPs proteome in HEK293 cells, mESCs and mouse organs

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Genome-wide mapping of PRDM15 binding in pluripotent mouse Embryonic Stem Cells (mESCs)

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