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Transcriptome sequencing of transgenic Nicotiana benthamiana producing moth pheromones [1]

ABSTRACT: Transcriptome sequencing of transgenic Nicotiana benthamiana producing moth pheromones [1]

PROVIDER: PRJNA790832 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17278600_1.fastq.gz	Fastqsanger.gz
	SRR17278600_2.fastq.gz	Fastqsanger.gz
	SRR17278601_1.fastq.gz	Fastqsanger.gz
	SRR17278601_2.fastq.gz	Fastqsanger.gz
	SRR17278602_1.fastq.gz	Fastqsanger.gz

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Transcriptome sequencing of transgenic Nicotiana benthamiana producing moth pheromones [2]

Project description:Insect sex pheromones are an important resource in integrated pest management. Encouraging steps have been made towards their sustainable production in plant biofactories, but important obstacles remain to be resolved. We have used transcriptomic analysis to determine the molecular perturbations behing growth penalty associated with high pheromone production in transgenic Nicotiana benthamiana lines with constitutive expression of genes for biosynthesis of moth sex pheromones - SexyPlants v1.2. We analyzed two lines with high production of pheromones as well as wt N. benthamiana.

2022-09-26 | GSE192368 | GEO

Transcriptome sequencing of transgenic Nicotiana benthamiana producing moth pheromones [1]

Project description:Insect sex pheromones are an important resource in integrated pest management. Encouraging steps have been made towards their sustainable production in plant biofactories, but important obstacles remain to be resolved. We have used transcriptomic analysis to determine the molecular perturbations behing growth penalty associated with high pheromone production in transgenic Nicotiana benthamiana lines with constitutive expression of genes for biosynthesis of moth sex pheromones - SexyPlants v1.0. We analyzed lines with high production (homozygous lines) and strong growth penalty as well as lines with low production (heterozygous lines) and negligible growth penalty.

2022-09-26 | GSE191269 | GEO

Transcriptome sequencing of transgenic Nicotiana benthamiana producing moth pheromones

Project description:Transcriptome sequencing of transgenic Nicotiana benthamiana producing moth pheromones

| PRJNA791299 | ENA

Transcriptome sequencing of transgenic Nicotiana benthamiana producing moth pheromones [2]

Project description:Transcriptome sequencing of transgenic Nicotiana benthamiana producing moth pheromones [2]

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Transcriptome sequencing of transgenic Nicotiana benthamiana producing moth pheromones

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-09-26 | GSE192369 | GEO

Nicotiana benthamiana

Project description:Nicotiana benthamiana

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Production of Volatile Moth Sex Pheromones in Transgenic <i>Nicotiana benthamiana</i> Plants.

Project description:Plant-based bioproduction of insect sex pheromones has been proposed as an innovative strategy to increase the sustainability of pest control in agriculture. Here, we describe the engineering of transgenic plants producing (Z)-11-hexadecenol (Z11-16OH) and (Z)-11-hexadecenyl acetate (Z11-16OAc), two main volatile components in many Lepidoptera sex pheromone blends. We assembled multigene DNA constructs encoding the pheromone biosynthetic pathway and stably transformed them into Nicotiana benthamiana plants. The constructs contained the Amyelois transitella AtrΔ11 desaturase gene, the Helicoverpa armigera fatty acyl reductase HarFAR gene, and the Euonymus alatus diacylglycerol acetyltransferase EaDAct gene in different configurations. All the pheromone-producing plants showed dwarf phenotypes, the severity of which correlated with pheromone levels. All but one of the recovered lines produced high levels of Z11-16OH, but very low levels of Z11-16OAc, probably as a result of recurrent truncations at the level of the EaDAct gene. Only one plant line (SxPv1.2) was recovered that harboured an intact pheromone pathway and which produced moderate levels of Z11-16OAc (11.8 μg g-1 FW) and high levels of Z11-16OH (111.4 μg g-1). Z11-16OAc production was accompanied in SxPv1.2 by a partial recovery of the dwarf phenotype. SxPv1.2 was used to estimate the rates of volatile pheromone release, which resulted in 8.48 ng g-1 FW per day for Z11-16OH and 9.44 ng g-1 FW per day for Z11-16OAc. Our results suggest that pheromone release acts as a limiting factor in pheromone biodispenser strategies and establish a roadmap for biotechnological improvements.

| S-EPMC10521740 | biostudies-literature

Small RNAs from Nicotiana benthamiana leaves

Project description:Small RNA expression from Nicotiana benthamiana leaves was profiled with the primary goal of ascertaining microRNA isoform diversity for known, conserved families. A secondary goal was to provide a baseline small RNA expression atlas for this species and tissue.

2014-02-10 | GSE48886 | GEO

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Project description:In order to find the differentially expressed RNAs and signaling pathways related to the dwarf phenotype induced by NlCSP11, 35S-NlCSP11, 35S-XopQ and 35S-GFP control were infiltrated into Nicotiana benthamiana. We analyzed the differentially expressed RNAs in the infiltrated leaves (NlCSP11_Z/XopQ_Z) and the upper uninfiltrated leaves (NlCSP11_U/XopQ_U) of N. benthamiana. KEGG annotation revealed a large number of differentially expressed RNAs involved in plant-pathogen interactions, indicating that NlCSP11 resulted in a more strong systemic resistance in N. benthamiana.

2024-05-30 | GSE267686 | GEO

Effect of Transcriptional-like Effectors (TALE) on Nicotiana benthamiana leaves

Project description:To investigate gene expression upon inoculation of Nicotiana benthamiana leaves with a TALE inducing cell death

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