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Effect of cyclic tensile stretch on meniscus cells in the presence and absence of IL-1

ABSTRACT: Effect of cyclic tensile stretch on meniscus cells in the presence and absence of IL-1

PROVIDER: PRJNA790976 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17282400_1.fastq.gz	Fastqsanger.gz
	SRR17282400_2.fastq.gz	Fastqsanger.gz
	SRR17282401_1.fastq.gz	Fastqsanger.gz
	SRR17282401_2.fastq.gz	Fastqsanger.gz
	SRR17282402_1.fastq.gz	Fastqsanger.gz

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Effect of cyclic tensile stretch on meniscus cells in the presence and absence of IL-1

Project description:Meniscus injuries are highly prevalent and are linked to the development of post-traumatic osteoarthritis (PTOA). The inflammatory cytokine IL-1 is elevated in synovial fluid following knee injuries, causes degradation of meniscus tissue, and inhibits meniscus repair. Cyclic tensile stretch has been shown to modulate the expression of pro-inflammatory cytokines and degradative enzymes induced by IL-1; however, there remains a dearth of knowledge on global effects of loading on meniscus cell phenotype and transcriptomic profiles. In this study, we performed mRNA-Seq on meniscus cells isolated from inner and outer zone regions of porcine menisci subjected to cyclic tensile strain in the presence and absence of IL-1 to identify cellular responses to mechanical load, identify differences in response to load based on zonal characteristics, and identify IL-1 induced inflammatory responses modulated by load.

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