Project description:Pathological processes like osteoporosis or steroid-induced osteonecrosis of the hip are accompanied by increased bone marrow adipogenesis. Such disorder of adipogenic/osteogenic differentiation, which affects also bone marrow derived mesenchymal stem cells (BMSCs) contributes to bone loss during aging. Therefore, we investigated the effects of extracellular vesicles (EVs) isolated from human (h)BMSCs during different stages of osteogenic differentiation on osteogenic and adipogenic differentiation capacity of naïve hBMSCs.
Project description:Small extracellular vesicles (sEV) play a pivotal role in intercellular communication and hold immense therapeutic potential. In this study, we aimed to characterize sEV derived from bone marrow mesenchymal stromal cells (BM-MSCs) and Wharton’s jelly mesenchymal stromal cells (WJ-MSCs), cultured under normoxic and hypoxic conditions for elucidating their functional inclinations. Using high-throughput miRNA sequencing, we identified distinct miRNA profiles across cell types and oxygenation states, revealing unique signatures associated with hypoxia-driven cellular adaptations.
Project description:Extracellular vesicles (EVs) released by human placenta–derived mesenchymal stem cells (hpMSCs) are thought to mediate paracrine effects. To characterize their small-RNA cargo, we isolated hpMSC-derived EVs (hpEVs) from conditioned media using size-exclusion chromatography and performed miRNA sequencing.
2025-11-03 | GSE305310 | GEO
Project description:miRNA sequencing of human mesenchymal stem cell-derived extracellular vesicles
| PRJNA814986 | ENA
Project description:miRNA sequencing of human mesenchymal stem cell-derived extracellular vesicles
Project description:miRNA-sequencing of grapefruit-derived extracellular vesicles and fusion nanovesicles derived from grapefruit-derived extracellular vesicles and gingival mesenchymal stem cell-derived vesicles. We then performed gene expression profiling analysis to explore the miRNAs derived from grapefruit-derived extracellular vesicles, and the retention rate of miRNAs after membrane fusion
Project description:To gain insight into the microRNA expression profile of small extracellular vesicles derived from bone metabolism related cell types and to verify their mechanism, we utilized the miRNA sequencing technology to analyze the miRNA profiles of different mouse osteoblast and osteoclast cell derived small extracellular vesicles.