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HPSC-Derived Enteric Ganglioids Model Human ENS Development and Function [snRNA-seq]

ABSTRACT: hPSC-Derived Enteric Ganglioids Model Human ENS Development and Function [snRNA-seq]

PROVIDER: PRJNA792546 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17334968_1.fastq.gz	Fastqsanger.gz
	SRR17334968_2.fastq.gz	Fastqsanger.gz
	SRR17334969_1.fastq.gz	Fastqsanger.gz
	SRR17334969_2.fastq.gz	Fastqsanger.gz
	SRR17334970_1.fastq.gz	Fastqsanger.gz

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hPSC-Derived Enteric Ganglioids Model Human ENS Development and Function [snRNA-seq]

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Maturing an Enteric Nervous System in Human Intestinal Organoid-derived Tissue-Engineered Small Intestine

Project description:Acquired or congenital disruption in enteric nervous system (ENS) development or function can lead to significant mechanical dysmotility. ENS restoration through cellular transplantation may provide a cure for enteric neuropathies. We have previously generated human pluripotent stem cell (hPSC)-derived tissue-engineered small intestine (TESI) from human intestinal organoids (HIO). However, HIO-TESI fails to develop an ENS. In a previous report of combined HIO with additional human enteric neural crest cells (ENCC), an ENS was established but lacked maturity. The purpose of our study is to establish a mature ENS derived exclusively from hPSC in HIO-TESI. hPSC-derived ENCC supplementation of HIO-TESI generates ENCC-HIO-TESI with mature submucosal and myenteric ganglia, repopulates excitatory, inhibitory, and sensory neurons, and restores the neuroepithelial circuit and neuron-dependent contractility and relaxation. Our findings validate a novel approach to restoring a functional hPSC-derived ENS in ENCC-HIO-TESI and implicate their potential for the treatment of enteric neuropathies.

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Project description:The enteric nervous system (ENS) can control most essential gut functions owing to its organization into complete neural circuits consisting of a multitude of different neuronal subtypes. We used microarrays to identify transcription factor networks and signaling pathways involved in diversification and differentiation of enteric neurons during development of the enteric nervous system.

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hPSC-Derived Enteric Ganglioids Model Human ENS Development and Function

Project description:This SuperSeries is composed of the SubSeries listed below.

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Loss of enteric neuronal Ndrg4 promotes colorectal cancer via increased release of Nid1 and Fbln2

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