Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Single-cell RNA-seq data for control and POU IV-misexpressed Ciona intestinalis embryos at late tailbud (LTB) stage

ABSTRACT: Single-cell RNA-seq data for control and POU IV-misexpressed Ciona intestinalis embryos at late tailbud (LTB) stage

PROVIDER: PRJNA792554 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR17334639_1.fastq.gz	Fastqsanger.gz
	SRR17334639_2.fastq.gz	Fastqsanger.gz
	SRR17334640_1.fastq.gz	Fastqsanger.gz
	SRR17334640_2.fastq.gz	Fastqsanger.gz

Items per page:

1 - 4 of 4

Similar Datasets

Single-cell RNA-seq data for control and POU IV-misexpressed Ciona intestinalis embryos at late tailbud (LTB) stage

Project description:Purpose: Understand how the misexpression of key sensory cell determinant POU IV affects cell type specification of various sensory neurons in the peripheral nervous system in Ciona. Methods: CesA>POU IV (2.5ng/l) + CesA>GFP (5.0ng/l) injected eggs and control eggs were fertilized side by side, and allowed to develop to the late tailbud stage (13.5h after fertilization at 18°C). For each sample, 120 morphologically normal embryos were used for single cell RNA-seq assays. Dissociation of the embryos and single cell RNA-seq assays by 10x Genomics chromium system were done as described in 9. Horie R. et al., Shared evolutionary origin of vertebrate neural crest and cranial placodes. Nature 560, 228-232 (2018). Results: Misexpression of POU IV leads to the formation of Synthetic Cell Types that exhibit properties of other sensory neurons, especially those of bipolar tail neurons (BTNs) and a synthetic gene battery that is not found in any wildtype sensory neuron.

2021-12-29 | GSE192645 | GEO

Single cell RNA seq for Ciona late tailbud embryos

Project description:We applied single cell RNA seq with 10x genomics platform to study the cell identity change in Ciona late tailbud embryos. By comparing single cell data from Pax3/7>Foxc amd control embryos, we found ectopic expression of FoxC in BTN cells transformed into PSC cells, which suggest common shared origin of BTNs and PSCs.

2018-07-31 | GSE115331 | GEO

Expression profiling of Ciona intestinalis neurula and mid-tailbud stage embryos expressing altered forms of the transcription factor Ci-Tbx2/3 in the notochord

Project description:Despite being found in the notochord of several chordates, the roles of the Tbx2 subfamily of T-box transcription factors in the development of this tissue remain largely unknown. We explored the targets of the only Tbx2 subfamily member in Ciona intestinalis, Ci-Tbx2/3, by expressing mutant forms of the transcriptional regulator using the Ci-Bra promoter region. We produced a dominant interfering version of Ci-Tbx2/3 (Tbx2/3DBD) through expression of a truncated version consisting only of its DNA-binding domain (DBD) and a constitutive activator form by attaching the T-box of Ci-Tbx2/3 to the VP16 transactivation domain (Tbx2/3VP16). These constructs were introduced into 1-cell stage embryos and grown to the neurula (N) or mid-tailbud (mTb) stage to capture targets regulated throughout notochord morphogenesis.

2013-04-27 | GSE42267 | GEO

Single cell RNA seq for Ciona late tailbud embryos

Project description:Single cell RNA seq for Ciona late tailbud embryos

| PRJNA474542 | ENA

Expression profiling of Ciona intestinalis neurula and mid-tailbud stage embryos expressing altered forms of the transcription factor Ci-Tbx2/3 in the notochord

2013-04-27 | E-GEOD-42267 | biostudies-arrayexpress

Ciona intestinalis (ascidian): control vs. TBT exposed

Project description:Transcriptional profiling of Ciona intestinalis comparing ethanol exposed asidian (control) with TBT exposed ascidian.

2010-07-20 | GSE22995 | GEO

Gene expression profiles of unfertilized eggs of Ciona intestinalis

Project description:The transcriptome of unfertilized eggs of Ciona intestinalis was determined with microarrays.

2013-10-29 | GSE45500 | GEO

small RNA sequencing of two developmental stages of Ciona intestinalis

Project description:We report the comprehensive sequencing of small RNA libraries created from different developmental stages (larva and gastrula) of the basal chordate, Ciona intestinalis. These libraries were used for the identification of microRNAs in this organism. Sequencing of small RNA libraries from 2 stages of Ciona intestinalis.

2010-03-27 | E-GEOD-21078 | biostudies-arrayexpress

Gene expression of ovary and neural complex in adult Ciona intestinalis

Project description:To compare with new Ciona intestinalis microarray NimbleGen 135k, we peformed gene expression analysis of GPL14686. Total RNAs from common samples with a part of NimbleGen's analysis are used for this analysis. Ovary and Neural Complex from adult Ciona intestinalis, duplicate, 2 time points during spawning

2011-11-05 | E-GEOD-33498 | biostudies-arrayexpress

Ciona intestinalis

Project description:Phagocytes of Ciona intestinalis

| PRJDB12544 | ENA