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Expression data from human lymphoblastoid cell lines (LCLs)

ABSTRACT: Expression data from human lymphoblastoid cell lines (LCLs)

PROVIDER: PRJNA793048 | ENA |

REPOSITORIES: ENA

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Expression data from human lymphoblastoid cell lines (LCLs)

Project description:The X-linked alpha thalassaemia intellectual disability syndrome (ATRX) protein is a member of the SWI/SNF family of chromatin remodelling factors which acts as an ATP dependent molecular motor. Germline mutations in ATRX give rise to a severe form of syndromal intellectual disability (ATR-X syndrome). To date, only a small number of genes have been identified that are affected by pathogenic ATRX mutations in human. We performed microarray experiments on LCLs from normal individuals and patients with diverse pathogenic ATRX mutations, to identify more genes regulated by ATRX.

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Expression analysis of Epstein-Barr virus–transformed human lymphoblastoid cell lines (LCLs) between male and female.

Project description:Investigation of human X-linked imprinted gene. Comparing pooled RNA of lymphoblastoid cell lines from normal human male and female, identify the genes expressed with sex spesific manner. Expression array study with total RNA extracted from pooled male and female LCLs with eight 60-mer probe per gene. 47633 exemplar genes representing a total of 63780 transcripts/variants.

2012-01-20 | E-GEOD-35207 | biostudies-arrayexpress

Transcription profiling of human lymphoblastoid cell lines (LCLs) derived from ICF syndrome and normal individuals.

Project description:Genome-wide DNA methylation patterns are established and maintained by the coordinated action of three DNA methyltransferases, DNMT1, DNMT3A, and DNMT3B. DNMT3B hypomorphic germline mutations are responsible for two-thirds of Immunodeficiency, Centromere Instability, Facial Anomalies (ICF) syndrome cases. The molecular defects in transcription, DNA methylation, and chromatin structure in ICF cells remain relatively uncharacterized. We used expressing microarrays to define the global program of gene expression to elucidate the role of DNMT3B in these processes using EBV-immortalized lymphoblastoid cell lines (LCLs) derived from ICF syndrome and normal individuals. Experiment Overall Design: 5 Normal LCLs (GM08729, GM08728, LCL1, CM304 and CM774) and 3 ICF LCLs (4088,GM08714 and 10759) were selected for RNA extraction and hybridization on Affymetrix UU133A/B microarrays.

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Gene expression profiling of human CD19+ B cells and EBV transformed lymphoblastoid cell lines (LCLs)

Project description:Gene expression profiling of human CD19+ B cells and EBV transformed lymphoblastoid cell lines (LCLs)

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RNA-seq: Analysis of differential gene expression between HD and DM1 patient lymphoblastoid cell lines (LCLs) compared to unaffected LCLs.

Project description:RNA-seq: Analysis of differential gene expression between HD and DM1 patient lymphoblastoid cell lines (LCLs) compared to unaffected LCLs.

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Expression analysis of Epstein-Barr virus–transformed human lymphoblastoid cell lines (LCLs) between male and female.

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Proteomics Analysis of Lymphoblastoid Cell lines from Patients with Amyotrophic Lateral Sclerosis

Project description:Amyotrophic lateral sclerosis (ALS) consists in the progressive degeneration of motor neurons, caused by mechanisms that are poorly understood and for which there is no cure. Some of the cellular perturbations associated with ALS can be detected in peripheral cells, including lym-phocytes from blood. A related cell system that is very suitable for research consists in human lymphoblastoid cell lines (LCLs), which are immortalized lymphocytes. LCLs that can be easily expanded in culture and can be maintained for long periods of time as stable cultures. We inves-tigated, on a small set of LCLs, if proteomics analysis by liquid chromatography followed by tandem mass spectrometry reveals proteins that are differentially present in ALS versus healthy controls. We found that individual proteins and cellular and molecular pathways in which these proteins participate, are detected as differentially present in the ALS samples. Some of these proteins and pathways are already known to be perturbed in ALS, while others are new and present interest for further investigations. These observations suggest that more detailed pro-teomics analysis of LCLs, using a larger number of samples, represents a promising approach for investigating ALS mechanisms and to search for therapeutic agents.

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The effect of freeze-thaw cycles on gene expression levels in lymphoblastoid cell lines

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Gene expression of Lymphoblastoid Cell Lines–LCLs from Depressed Patients after in-vitro treatment with citalopram–CTP

Project description:Gene expression of Lymphoblastoid Cell Lines–LCLs from Depressed Patients after in-vitro treatment with citalopram–CTP

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RNA-seq: Analysis of differential gene expression between HD and DM1 patient lymphoblastoid cell lines (LCLs) compared to unaffected LCLs.

Project description:Transcriptome from HD, DM1, and unaffected LCLs.

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