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Bradyrhizobium japonicum

ABSTRACT: Bacteriostatic-resistant Bradyrhizobium japonicum E109 Raw sequence reads

PROVIDER: PRJNA793169 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17388438_1.fastq.gz	Fastqsanger.gz
	SRR17388438_2.fastq.gz	Fastqsanger.gz
	SRR17382844_1.fastq.gz	Fastqsanger.gz
	SRR17382844_2.fastq.gz	Fastqsanger.gz
	SRR17385245_1.fastq.gz	Fastqsanger.gz

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Bradyrhizobium japonicum strain:E109 | isolate:E1

Project description:Oxidative stress-resistant Bradyrhizobium japonicum E109 Raw sequence reads

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Bradyrhizobium japonicum strain:E109

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Comparison of Bradyrhizobium japonicum parent strain LO with irr mutant strain LODTM5

Project description:The purpose of the study is to identify Irr-responsive genes in the bacterium Bradyrhizobium japonicum. Parent strain LO was compared to irr mutant strain LODTM5 by whole genome microarray analysis. Both cell types were grown in iron-limited media. Keywords: Comparison of B. japonicum wild type and mutant cells

2006-03-15 | GSE4155 | GEO

Global consequences of phosphatidylcholine reduction in Bradyrhizobium japonicum

Project description:Analysis of a Bradyrhizobium japonicum pmtA mutant. PmtA catalyzes the first of three consecutive methylation reactions leading to phosphatidylcholine (PC) formation in B. japonicum. Disruption of the pmtA gene results in a significantly reduced PC content causing a defect in symbiosis with the soybean host. This study provides the first insight into global transcriptomic changes of a bacterial phosphatidylcholine biosynthesis mutant. Cells of the pmtA mutant and the wild type were grown to mid-exponential phase in full medium (PSY) under aerobic culture conditions. Keywords: genetic modification

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Global consequences of phosphatidylcholine reduction in Bradyrhizobium japonicum

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Characterization of the Bradyrhizobium japonicum NtrC regulon by DNA Microarray analysis.

2012-07-01 | GSE23870 | GEO

Characterization of the Bradyrhizobium japonicum NtrC regulon by DNA Microarray analysis.

2015-06-25 | GSE66091 | GEO

Bradyrhizobium japonicum proteomic reference map, and Vigna mungo leaf proteome map

Project description:DISCLAIMER: This project actually contains two separate and independent assays by mistake. They should be not be considered together.</br></br>Assay 9769 - Bradyrhizobium japonicum proteomic reference map PMID : 20806226</br>Assay 15318 - Vigna mungo leaf proteome map PMID : 23587433

2011-11-15 | PRD000119 | Pride

Characterization of the Bradyrhizobium japonicum NtrC regulon by DNA Microarray analysis.

Project description:The Bradyrhizobium japonicum NtrC regulatory protein influences gene expression in response to changes in intracellular nitrogen status. Under conditions of low nitrogen, phosphorylation of NtrC results in up-regulation of a number of genes involved in nitrogen metabolism and nitrogen acquisition. To better define the exact nature of NtrC’s influence on gene expression, a ntrC mutation was created in B. japonicum and transcriptional profiling was performed by DNA microarray analysis of both the mutant and wild type strains. Bradyrhizobium japonicum USDA 110 and a ntrC mutant in the USDA 110 background were cultured in minimal medium supplemented with either 10mM glutamate (low nitrogen) or 10mM ammonium and 10mM glutamate (high nitrogen) as nitrogen sources. Four comparisons were performed: wild type high nitrogen vs. mutant high nitrogen, wild type low nitrogen vs. wild type high nitrogen, wild type low nitrogen vs. mutant low nitrogen, and mutant low nitrogen vs. mutant high nitrogen. For each of the four comparisons, three biological replicates were prepared for each strain and dye swap replications were performed for each hybridization producing a total of six arrays per comparison and 24 arrays in total.

2015-06-25 | E-GEOD-66091 | biostudies-arrayexpress

Transcription profiling of soybean nodulation by Bradyrhizobium japonicum

Project description:Legumes interact with nodulating bacteria that convert atmospheric nitrogen into ammonia for plant use. This nitrogen fixation takes place within root nodules that form after infection of root hairs by compatible rhizobia. Using cDNA microarrays, we monitored gene expression in soybean (Glycine max) inoculated with the nodulating bacterium Bradyrhizobium japonicum 4, 8, and 16 days after inoculation (dai), time points that coincided with nodule development and the onset of nitrogen fixation. This experiment identified several thousand genes that were differentially expressed in response to B. japonicum inoculation. Expression of 27 genes was analyzed by qRT-PCR and their expression patterns mimicked the microarray results confirming integrity of analyses. The microarray results suggest that B. japonicum reduces plant defense responses during nodule development. In addition, the data revealed a high level of regulatory complexity (transcriptional, post-transcriptional, translational, post-translational) that is likely essential for development of the symbiosis and adjustment to an altered nutritional status. Keywords = symbiosis Keywords = nodulation Keywords = rhizobium Keywords = defense Keywords = ANOVA Keywords = plant Keywords: nodulating vs not nodulating

2008-02-05 | GSE10340 | GEO

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