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Next generation sequencing compares CNS-infiltrating CD4 cells versus draining lymph node effector CD4 cells in experimental autoimmune encephalomyelitis

ABSTRACT: Next generation sequencing compares CNS-infiltrating CD4 cells versus draining lymph node effector CD4 cells in experimental autoimmune encephalomyelitis

PROVIDER: PRJNA793585 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17407538_1.fastq.gz	Fastqsanger.gz
	SRR17407538_2.fastq.gz	Fastqsanger.gz
	SRR17407539_1.fastq.gz	Fastqsanger.gz
	SRR17407539_2.fastq.gz	Fastqsanger.gz

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Next generation sequencing compares CNS-infiltrating CD4 cells versus draining lymph node effector CD4 cells in experimental autoimmune encephalomyelitis

Project description:The immunology has been advanced mostly by studying peripheral blood in human and peripheral immune organ in mouse. However, a long standing problem in develop therapeutics treating autoimmune diseases is that we are short of knowledge about inflammatory-tissue infiltraing CD4 cells, which are terminally differentiated effector that exactly mediate the tissue damage. Thus, to address this issue, we induced experimental autoimmune encephalomyelitis (EAE) in mouse and sorted out both CNS infiltrating CD4 cells and draining lymph node effector CD4 cells at disease onset, and compared their transcriptome

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Quantitative proteome profiling of CNS-infiltrating autoreactive CD4+ cells reveals selective changes during experimental autoimmune encephalomyelitis

Project description:Experimental autoimmune encephalomyelitis (EAE) is a murine model of multiple sclerosis, a chronic neurodegenerative and inflammatory autoimmune condition of the central nervous system (CNS). Pathology is driven by the infiltration of autoreactive CD4+ lymphocytes into the CNS where they attack neuronal sheaths causing ascending paralysis. We used an isotope-coded protein labelling approach to investigate the proteome of CD4+ cells isolated from the spinal cord and brain of mice at various stages of EAE progression in two EAE disease models; PLP139-151-induced relapsing-remitting EAE and MOG35-55-induced chronic EAE, which emulate the two forms of human multiple sclerosis. A total of 1120 proteins were quantified across disease onset, peak-disease and remission phases of disease and of these, 13 up-regulated proteins of interest were identified with functions relating to the regulation of inflammation, leukocyte adhesion and migration, tissue repair and the regulation of transcription/translation. Proteins implicated in processes such as inflammation (S100A4 and S100A9) and tissue repair (Annexin A1), which represent key events during EAE progression were validated by quantitative PCR. This is the first targeted analysis of autoreactive cells purified from the CNS during EAE, highlighting fundamental CD4+ cell-driven processes that occur during the initiation of relapse and remission stages of disease.

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