Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Mechanical Unloading of Engineered Human Meniscus Models Under Simulated Microgravity

ABSTRACT: Mechanical Unloading of Engineered Human Meniscus Models Under Simulated Microgravity

PROVIDER: PRJNA794056 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR17423000_1.fastq.gz	Fastqsanger.gz
	SRR17423000_2.fastq.gz	Fastqsanger.gz
	SRR17423001_1.fastq.gz	Fastqsanger.gz
	SRR17423001_2.fastq.gz	Fastqsanger.gz
	SRR17423002_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 32

Similar Datasets

Mechanical Unloading of Engineered Human Meniscus Models Under Simulated Microgravity

Project description:The aim of this study is to demonstrate that mechanical unloading via SMG will induce a higher osteoarthritic-like gene profile in bioengineered meniscal cartilage from healthy female MFC versus healthy male MFC. This would serve as the molecular basis for early onset of knee osteoarthritis in females

2022-05-20 | GSE192983 | GEO

Changes in nuclear shape and gene expression in response to simulated microgravity are LINC complex-dependent

Project description:Microgravity is known to affect the organization of the cytoskeleton, cell and nuclear morphology and to elicit differential expression of genes associated with the cytoskeleton, focal adhesions and the extracellular matrix. Although the nucleus is mechanically connected to the cytoskeleton through the LInker of Nucleoskeleton and Cytoskeleton (LINC) complex, the role of this group of proteins in these responses to microgravity has yet to be defined. Therefore, we used simulated microgravity achieved by growing cells on a 3d clinostat to investigate whether the LINC complex acts to mediate responses to the microgravity environment. We show that nuclear shape and differential gene expression are both responsive to simulated microgravity in a LINC-dependent manner and that this response changes with the duration of exposure to simulated microgravity. These LINC-dependent genes likely represent elements normally regulated by the mechanical forces imposed by gravity on Earth.

2021-01-31 | GSE153893 | GEO

RNA sequencing of zebrafish embryos under simulated microgravity conditions

Project description:To reveal the potential mechanisms involved in the dysfunction of antiviral immune responses under simulated microgravity conditions, we investigated the transcriptional changes related to the status of innate immune responses by RNA-seq with poly I:C or mock PBS treatment under Normal gravity or simulated microgravity conditions. Our results indicate that the retinoic acid inducible gene (RIG)-I-like receptor (RLR) and Toll-like receptor (TLR) signal pathways, which are both involved in the type-I interferon induction, are significantly inhibited by simulated microgravity effects.

2020-12-22 | GSE145967 | GEO

Human Peripheral Blood Lymphcoyte(PBL): Simulated Microgravity vs. Control

Project description:Transcriptional profiling of human peripheral blood lymphocyte comparing simulated microgravity for 72 hours with untreated control. Two-condition experiment, simulated microgravity vs.untreated control. Biological replicates: 1 control, 1 treated, independently grown and harvested.

2014-04-10 | E-GEOD-56647 | biostudies-arrayexpress

static vs simulated microgravity

Project description:The below table includes a smaller list of data that was analyzed by dChip and filtered by pvalue such that a file with about 4600 genes was obtained, which allowed for ease of use from 40,000 genes. Keywords: static vs simulated microgravity

2006-12-31 | GSE4658 | GEO

Limnospira indica PCC8005 in simulated microgravity

Project description:Regenerative life support systems for space crews recycle organic and inorganic waste into water, food and oxygen using different organisms. For instance, the European Space Agency's MELiSSA uses Limnospira indica PCC8005 for air revitalisation and food production. Before use in space, the components' compatibility with reduced gravity must be tested. This innovative study introduces a novel ground analog designed specifically for microgravity experiments involving cyanobacteria, employing a cutting-edge random positioning machine (RPM). Limnospira indica PCC8005 was shown to grow slower under simulated microgravity and whole proteome analysis revealed a downregulation of e.g. ribosomal proteins, glutamine synthase and nitrate uptake transporters while an upregulation was found for gas vesicle proteins, carboxysome proteins and phycobiliproteins. All together our results suggested that L. indica experienced carbon limitation when cultivated in simulated microgravity conditions.

2024-12-19 | PXD045600 | Pride

Human Peripheral Blood Lymphcoyte(PBL): Simulated Microgravity vs. Control

Project description:Transcriptional profiling of human peripheral blood lymphocyte comparing simulated microgravity for 72 hours with untreated control.

2014-04-10 | GSE56647 | GEO

Engineered human meniscus in modeling sex differences of knee osteoarthritis in vitro

Project description:This study aimed to evaluate the effects of mechanical loading and unloading via CHP and SMG, respectively, on the OA-related profile changes of engineered meniscus tissues and explore biological sex-related differences

2022-01-26 | GSE192982 | GEO

Effects of Simulated Microgravity on Colorectal Cancer Organoids Growth and Drug Response

Project description:Cellular and molecular dynamics of human cells are constantly affected by gravity. Alteration of the gravitational force disturbs the cellular equilibrium, which might modify physiological and molecular characteristics. Nevertheless, biological responses of cancer cells to reduced gravitational force remains obscure. Here, we aimed to comprehend not only transcriptomic patterns but drug responses of colorectal cancer (CRC) under simulated microgravity. We established four organoids directly from CRC patients, and organoids cultured in 3D clinostat were subjected to genome wide expression profiling and drug library screening. Our observations revealed changes in cell morphology and an increase in cell viability under simulated microgravity compared to their static controls. Transcriptomic analysis highlighted a significant dysregulation in the TBC1D3 family of genes. The upregulation of cell proliferation observed under simulated microgravity conditions was further supported by enriched cell cycle processes, as evidenced by the functional clustering of mRNA expressions using cancer hallmark and gene ontology terms. Our drug screening results indicated an enhanced response rate to 5-FU under conditions of simulated microgravity, suggesting potential implications for cancer treatment strategies in simulated microgravity.

2024-09-11 | GSE271642 | GEO

Streptococcus mutans differential gene expression in response to simulated microgravity

Project description:Astronauts have been previously shown to exhibit decreased salivary lysozyme and increased dental calculus and gingival inflammation in response to space flight, host factors that could contribute to oral diseases such as caries and periodontitis. However, the specific physiological response of caries-causing bacteria such as Streptococcus mutans to space flight and/or ground-based simulated microgravity has not been extensively investigated. In this study, High Aspect Ratio Vessel (HARV) S. mutans simulated microgravity and normal gravity cultures were assessed for changes in metabolite and transcriptome profiles, H2O2 resistance, and competence in sucrose-containing biofilm media. Stationary phase S. mutans simulated microgravity cultures displayed increased killing by H2O2 compared to normal gravity control cultures, but competence was not affected. RNA-seq analysis revealed that expression of 153 genes was up-regulated ≥ 2-fold and 94 genes down-regulated ≥ 2-fold during simulated microgravity HARV growth. These included a number of genes located on extrachromosomal elements, as well as genes involved in carbohydrate metabolism, translation, and stress responses. Collectively, these results suggest that growth under microgravity analog conditions promotes changes in S. mutans gene expression and physiology that may translate to an altered cariogenic potential of this organism during space flight missions.

2017-03-01 | GSE90166 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data