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Orthogonally induced differentiation of stem cells for the pattering of vascularized organoids and bioprinted tissues

ABSTRACT: Orthogonally induced differentiation of stem cells for the pattering of vascularized organoids and bioprinted tissues

PROVIDER: PRJNA795138 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17465700_1.fastq.gz	Fastqsanger.gz
	SRR17465700_2.fastq.gz	Fastqsanger.gz
	SRR17465701_1.fastq.gz	Fastqsanger.gz
	SRR17465701_2.fastq.gz	Fastqsanger.gz
	SRR17465702_1.fastq.gz	Fastqsanger.gz

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Orthogonally induced differentiation of stem cells for the pattering of vascularized organoids and bioprinted tissues [bulkRNA-seq]

Project description:Simultaneous differentiation of human induced pluripotent stem cells (hiPSCs) into divergent cell types offers a pathway to achieving tailorable cellular complexity, patterned architecture, and function in engineered human organoids and tissues. Recent transcription factor (TF) overexpression protocols typically produce a single cell type of interest rather than the multitude of cell types and structural organization found in native human tissues. Here, we report an orthogonal induced differentiation platform, wherein pluripotent cells are simultaneously co-differentiated into distinct cell types to generate organoids and bioprinted tissues with controlled composition and organization. To demonstrate this platform, we differentiated endothelial cells and neurons from hiPSCs in a one-pot system containing either neural or endothelial stem cell-specifying media. By aggregating inducible-TF and wild type hiPSCs into pooled and multicore-shell embryoid bodies, we produced vascularized and patterned cortical organoids within days. Using multimaterial 3D bioprinting, we patterned 3D neural tissues from densely cellular, matrix-free stem cell inks that underwent orthogonal induced differentiation to generate distinct layered regions composed of neural stem cells, endothelium, and neurons, respectively. Given the high proliferative capacity and patient-specificity of hiPSCs, our platform provides a facile route for programming cells and multicellular tissues for drug screening and therapeutic applications.

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Orthogonally induced differentiation of stem cells for the pattering of vascularized organoids and bioprinted tissues [scRNA-seq]

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Orthogonally induced differentiation of stem cells for the pattering of vascularized organoids and bioprinted tissues [bulkRNA-seq]

Project description:Orthogonally induced differentiation of stem cells for the pattering of vascularized organoids and bioprinted tissues [bulkRNA-seq]

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Orthogonally induced differentiation of stem cells for the pattering of vascularized organoids and bioprinted tissues [scRNA-seq]

Project description:Orthogonally induced differentiation of stem cells for the pattering of vascularized organoids and bioprinted tissues [scRNA-seq]

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High-speed live cell interferometry for screening bioprinted organoids

Project description:We developed bioprinted tumor organoids linked to real-time growth pattern quantitation via high-speed live cell interferometry (HSLCI). We demonstrate that bioprinting gives rise to 3D organoid structures that preserve histology and gene expression.

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Project description:Bulk RNA-seq comparison of kidney organoids bioprinted in 3 different conformations with varying starting cell densities. Density is dictated by the ratio of bioprinter tip movement to the amount of extrusion, where higher ratios spread cells over a larger surface area. We compare organoids printed with no movement ('blob', ratio 0) to those with moderate ('line 3', ratio 20) or high movement ('line 1', ratio 40).

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Project description:Comparison of bioprinted kidney organoids in different conformations

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