Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

CITE-seq of CD4+ T and myeloid cells in MS Twin cohort.

ABSTRACT: CITE-seq of CD4+ T and myeloid cells in MS Twin cohort.

PROVIDER: PRJNA795192 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR17469300_1.fastq.gz	Fastqsanger.gz
	SRR17469300_2.fastq.gz	Fastqsanger.gz
	SRR17469400_1.fastq.gz	Fastqsanger.gz
	SRR17469400_2.fastq.gz	Fastqsanger.gz
	SRR17469500_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 1122

Similar Datasets

CITE-seq of CD4+ T and myeloid cells in MS Twin cohort.

Project description:By integrating data on the immune profiles of healthy monozygotic and dizygotic twin pairs we estimated the variance in CD25 expression by naïve Th cells to be largely driven by genetic and shared early environmental influences. Nonetheless, the Th cell subset expanded in MS twins, which was also elevated in non-twin MS patients , emerged independent of the individual genetic makeup. These cells expressed CNS-homing receptors, exhibited a dysregulated CD25-IL-2 axis, and their proliferative capacity positively correlated with MS severity . Together, the pair-matched analysis of the extended twin approach allowed us to discern genetically- and environmentally- determined features of an MS-associated immune signature.

2022-01-13 | GSE193181 | GEO

CSF CD4+ T cells in MS

Project description:CSF CD4+ T cells in MS

| PRJNA721683 | ENA

Gene expression profile in CD4+ and CD8+ T cells from identical twins discordant for Multiple sclerosis

Project description:To gain insight into the etiopathogenesis of Multiple sclerosis (MS) we investigated gene expression changes in CD4+ and CD8+ T lymphocytes from monozygotic twins (MZ) discordant for relapsing remitting MS. We studied 4 monozygotic twin pairs discordant for disease, with the affected co-twin free of disease modifying therapies (F/M = 3/1, mean age 36.25±3.9). Following leukapheresis, CD4+ and CD8+ T cells were separated and studied by Affymetrix GeneChip®

2010-12-31 | GSE16461 | GEO

Twin study identifies early immunological and metabolic dysregulation of CD8+ T cells in multiple sclerosis

Project description:Multiple sclerosis (MS) is an inflammatory neurological disease of the central nervous system with a subclinical phase preceding frank neuroinflammation. CD8+ T cells are abundant within MS lesions, but their potential role in disease pathology remains unclear. Using high-throughput single-cell RNA sequencing and single-cell T cell receptor analysis, we compared CD8+ T cell clones from the blood and cerebrospinal fluid (CSF) of monozygotic twin pairs in which the co-twin had either no or subclinical neuroinflammation (SCNI). We identified peripheral MS-associated immunological and metabolic alterations indicative of an enhanced migratory, pro-inflammatory, and activated CD8+ T cell phenotype, which was also evident in co-twins with SCNI and in an independent validation cohort of people with MS (pwMS). Together, our in-depth single-cell analysis indicates a disease-driving pro-inflammatory role of infiltrating CD8+ T cells and identifies potential immunological and metabolic therapeutic targets in both prodromal and definitive stages of the disease.

2024-09-26 | GSE276167 | GEO

CITE-seq reveals inhibition of NF-kB pathway in B cells from vitamin D-treated multiple sclerosis patients

Project description:Vitamin D deficiency is a risk factor for multiple sclerosis (MS) and is correlated with disease activity and progression. Vitamin D treatment has emerged as potentially protective, even though results from randomized controlled trials are conflicting. Here, we used single-cell RNA-seq (scRNA-seq) coupled with barcoded antibodies targeting surface markers (CITE-seq) to discover candidate genes and pathways regulated in PBMC subpopulations from MS patients treated with high-dose vitamin D (n=5) or placebo (n=5). Best candidates were combined with genes involved in immune function and vitamin D metabolism for validation in a new cohort (n=8 in each group) by high-throughput qPCR (HT-qPCR) in naive CD4, Th1, Th17, Treg, naive CD8, memory and naive B cells, and MAIT cells, sorted by FACS. CITE-seq showed no significant changes in the proportions of these subpopulations in vitamin D-treated patients. Of the 92 candidate genes revealed by CITE-seq, differential expression of five genes (UXT, SNRPN, SUB1, GNLY and KLF6) was validated by HT-qPCR. CITE-seq also revealed the regulation of several pathways by vitamin D in naive and memory B cells, including MAPK, TLR and interleukin pathways, that contribute to counteract EBV-induced resistance to apoptosis through inhibition of the NF-kB pathway.

2023-10-05 | GSE239626 | GEO

twin

Project description:Drosophila melanogaster twin, twin, is differentially expressed in 17 experiment(s);

| FBgn0011725 | ExpressionAtlas

twin

Project description:Drosophila melanogaster twin, twin, is expressed in 3 baseline experiment(s);

| FBgn0011725 | ExpressionAtlas

CD4

Project description:Monodelphis domestica CD4, Monodelphis domestica CD4 molecule (CD4), mRNA. [Source:RefSeq mRNA;Acc:NM_001099290], is expressed in 2 baseline experiment(s);

| ENSMODG00000027359 | ExpressionAtlas

scRNAseq of blood and CSF CD4+ T cells from MS patients and controls

Project description:To study the regulation of candidate genes from our study in human cells, we analyzed CD4+ T cells from blood and CSF of MA patients and age and sex matched idiopathic intracranial hypertension controls We analyzed 40845 cells in control blood, 807 cells in control CSF, 29749 cells in MS blood and 15768 cells in MS CSF

2023-08-01 | GSE232343 | GEO

Cd4

Project description:Rattus norvegicus Cd4, Cd4 molecule [Source:RGD Symbol;Acc:2306], is expressed in 2 baseline experiment(s);

| ENSRNOG00000016294 | ExpressionAtlas