Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Oncopeltus fasciatus

ABSTRACT: The maternal and early embryonic transcriptome of the milkweed bug Oncopeltus fasciatus

PROVIDER: PRJNA79671 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR057570.fastq.gz	Fastqsanger.gz
	SRR057571.fastq.gz	Fastqsanger.gz
	SRR057572.fastq.gz	Fastqsanger.gz
	SRR057573.fastq.gz	Fastqsanger.gz

Items per page:

1 - 4 of 4

Similar Datasets

Oncopeltus fasciatus

Project description:Oncopeltus fasciatus embryonic transcriptomes

| PRJNA1171382 | ENA

Oncopeltus fasciatus

Project description:Oncopeltus fasciatus overview

| PRJNA60597 | ENA

Oncopeltus fasciatus

Project description:Oncopeltus fasciatus RNAseq

| PRJNA275739 | ENA

Oncopeltus fasciatus

Project description:Oncopeltus fasciatus Transcriptome or Gene expression

| PRJNA302284 | ENA

Oncopeltus fasciatus

Project description:Oncopeltus fasciatus genome sequencing project

| PRJNA60465 | ENA

Oncopeltus fasciatus

Project description:Whole genome assembly of Oncopeltus fasciatus using multiple sequencing technologies.

| PRJNA229125 | ENA

Post-transcriptional knockdown of Dnmt1 demonstrates a functional role for insect cytosine methylation

Project description:Despite considerable speculation for the role of cytosine (DNA) methylation in biological and molecular processes in insects, direct functional tests are lacking. Here we provide evidence for the functional role of the maintenance DNA methyltransferase 1 (Dnmt1) in an insect using experimental manipulation. Through RNA interference (RNAi) we successfully post-transcriptionally knocked down Dnmt1 in ovarian tissue of the hemipteran Oncopeltus fasciatus (the large milkweed bug). Individuals depleted for dnmt1, and subsequently DNA methylation, failed to reproduce. Manipulating the levels of DNA methylation did not result in changes in overall gene expression. Furthermore, reductions in levels of DNA methylation at transposable elements (TEs) did not lead to large-scale reactivation of TE transcription. Despite the lack of a causal relationship between reduced DNA methylation and gene expression in the tissue we surveyed, eggs were inviable revealing an important function of DNA methylation in O. fasciatus. Our work provides direct experimental evidence for a functional role of Dnmt1 and DNA methylation in insects and presents O. fasciatus as a tractable model for further exploration of the function of DNA methylation in other tissues and life history circumstances for insects.

2018-07-05 | GSE109199 | GEO

Functional anylsis of Oncopeltus fasciatus Na,K-ATPase genes

Project description:Expression of Oncopeltus fasciatus Na,K-ATPase alpha and beta subunits in cell culture

| PRJEB50512 | ENA

Oplegnathus fasciatus

Project description:Oplegnathus fasciatus gonad transcriptome

| PRJNA295471 | ENA

Oplegnathus fasciatus

Project description:Oplegnathus fasciatus gonad transcriptome

| PRJNA300396 | ENA