Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene silencing dynamics are modulated by transiently active regulatory elements

ABSTRACT: Gene silencing dynamics are modulated by transiently active regulatory elements

PROVIDER: PRJNA798037 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR17621855.fastq.gz	Fastqsanger.gz
	SRR17621856.fastq.gz	Fastqsanger.gz
	SRR17621857.fastq.gz	Fastqsanger.gz
	SRR17622757_1.fastq.gz	Fastqsanger.gz
	SRR17622757_2.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 126

Similar Datasets

Gene silencing dynamics are modulated by transiently active regulatory elements [ChIP-seq]

Project description:Gene silencing dynamics are modulated by transiently active regulatory elements [ChIP-seq]

| PRJNA797444 | ENA

Gene silencing dynamics are modulated by transiently active regulatory elements [4C-seq]

Project description:Gene silencing dynamics are modulated by transiently active regulatory elements [4C-seq]

| PRJNA797381 | ENA

Gene silencing dynamics are modulated by transiently active regulatory elements [PRO-seq]

Project description:Gene silencing dynamics are modulated by transiently active regulatory elements [PRO-seq]

| PRJNA797443 | ENA

Gene silencing dynamics are modulated by transiently active regulatory elements [ChIP-seq II]

Project description:Gene silencing dynamics are modulated by transiently active regulatory elements [ChIP-seq II]

| PRJNA925473 | ENA

Gene silencing dynamics are modulated by transiently active regulatory elements [ChIP-seq]

Project description:Transcriptional enhancers have been extensively characterized, but cis-regulatory elements involved in acute gene repression have received less attention. Transcription factor GATA1 promotes erythroid differentiation by activating and repressing distinct gene sets. Here, we study the mechanism by which GATA1 silences the proliferative gene Kit during erythroid maturation, and define stages from initial loss of activation to heterochromatinization. We find that GATA1 inactivates a potent upstream enhancer but concomitantly creates a discrete intronic regulatory region marked by H3K27ac, short noncoding RNAs, and de-novo chromatin looping. This enhancer-like element forms transiently and serves to delay Kit silencing. The element is ultimately erased via engagement of the FOG1/NuRD deacetylase complex, as revealed by study of a disease-associated GATA1 variant. Hence, regulatory sites can be self-limiting by dynamic co-factor usage. Genome-wide analyses across cell types and species uncover transiently active elements at numerous genes during repression, suggesting that modulation of silencing kinetics is widespread.

2023-02-14 | GSE193740 | GEO

Gene silencing dynamics are modulated by transiently active regulatory elements [4C-seq]

2023-02-14 | GSE193718 | GEO

Gene silencing dynamics are modulated by transiently active regulatory elements [PRO-seq]

2023-02-14 | GSE193741 | GEO

Gene silencing dynamics are modulated by transiently active regulatory elements [ChIP-seq II]

2023-02-14 | GSE223244 | GEO

Gene silencing dynamics are modulated by transiently active regulatory elements.

Project description:Transcriptional enhancers have been extensively characterized, but cis-regulatory elements involved in acute gene repression have received less attention. Transcription factor GATA1 promotes erythroid differentiation by activating and repressing distinct gene sets. Here, we study the mechanism by which GATA1 silences the proliferative gene Kit during murine erythroid cell maturation and define stages from initial loss of activation to heterochromatinization. We find that GATA1 inactivates a potent upstream enhancer but concomitantly creates a discrete intronic regulatory region marked by H3K27ac, short noncoding RNAs, and de novo chromatin looping. This enhancer-like element forms transiently and serves to delay Kit silencing. The element is ultimately erased via the FOG1/NuRD deacetylase complex, as revealed by the study of a disease-associated GATA1 variant. Hence, regulatory sites can be self-limiting by dynamic co-factor usage. Genome-wide analyses across cell types and species uncover transiently active elements at numerous genes during repression, suggesting that modulation of silencing kinetics is widespread.

| S-EPMC10719944 | biostudies-literature

Gene silencing dynamics are modulated by transiently active regulatory elements

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-02-14 | GSE193825 | GEO