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Time course of ethylene and ACC response in ETR1 receptor mutants in roots

ABSTRACT: Time course of ethylene and ACC response in ETR1 receptor mutants in roots

PROVIDER: PRJNA798052 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17646300_1.fastq.gz	Fastqsanger.gz
	SRR17646300_2.fastq.gz	Fastqsanger.gz
	SRR17646301_1.fastq.gz	Fastqsanger.gz
	SRR17646301_2.fastq.gz	Fastqsanger.gz
	SRR17646302_1.fastq.gz	Fastqsanger.gz

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Time course of ethylene and ACC response in ETR1 receptor mutants in roots

Project description:ETR1 receptor mutants etr1-3 (loss-of-function) and etr1-7 (gain-of-function) were treated with either ethylene or its biosynthetic precursor, ACC (1-aminocyclopropane-1-carboxylate) for 0, 1, 4, and 24 hours.

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Project description:The purpose of this experiment was to study the effects of the bacterial enzyme ACC deaminase on the transcriptional changes within canola seedlings. Seedlings from seeds treated with the plant growth-promoting bacteria Pseudomonas putida UW4 which expresses a high level of ACC deaminase and its ACC deaminase-minus mutant were compared to untreated seedlings along with a transgenic line of canola expressing the ACC deaminase enzyme in the roots. ACC deaminase breaks down 1-aminocyclopropane-1-carboxylic acid, the biosynthetic precursor to the plant hormone ethylene, lowering ethylene levels and improving plant fitness. Plants treated with the ACC deaminase-containing bacteria and transgenic plants expressing ACC deaminase are more tolerant to a variet of stresses and this expression study helps to illuminate the pathways responsible for the growth promotion provided by the beneficial bacteria and the role of the enzyme itself.

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