Project description:We used CCK-8 experiment to determine the chemotherapy tolerance of small cell lung cancer cells, from which we found chemotherapy-sensitive cells (H446) and chemotherapy-tolerant cells (SHP77).By RNA-SEQ, we found 99 miRNAs with abnormal expression associated with chemotherapy tolerance, including 69 up-regulated miRNAs and 30 down-regulated miRNAs. Several miRNAs related to chemotherapy tolerance of small cell lung cancer were found through qRT-PCR verification, which helped us to further clarify the mechanism of chemotherapy tolerance of small cell lung cancer.
Project description:We used CCK-8 experiment to determine the chemotherapy tolerance of small cell lung cancer cells, from which we found chemotherapy-sensitive cells (H446) and chemotherapy-tolerant cells (SHP77). By RNA-SEQ, we found 972 mRNAs with abnormal expression associated with chemotherapy tolerance, including 560 up-regulated mRNAs and 412 down-regulated mRNAs. Several miRNAs related to chemotherapy tolerance of small cell lung cancer were found through qRT-PCR verification, which helped us to further clarify the mechanism of chemotherapy tolerance of small cell lung cancer.
Project description:We used CCK-8 experiment to determine the chemotherapy tolerance of small cell lung cancer cells, from which we found chemotherapy-sensitive cells (H446) and chemotherapy-tolerant cells (SHP77).By RNA-SEQ, we identified 401 circRNAs with abnormal expression associated with chemotherapy tolerance, including 212 up-regulated circRNAs and 189 down-regulated circRNAs. Through qRT-PCR verification, several circRNAs related to chemotherapy tolerance of small cell lung cancer were found, which helped us to further clarify the mechanism of chemotherapy tolerance of small cell lung cancer.
