Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Insm1 regulates the expression of thymic tissue-restricted antigens and the immune tolerance [CUT&Tag]

ABSTRACT: Insm1 regulates the expression of thymic tissue-restricted antigens and the immune tolerance [CUT&Tag]

PROVIDER: PRJNA798410 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR23025687_1.fastq.gz	Fastqsanger.gz
	SRR23025687_2.fastq.gz	Fastqsanger.gz
	SRR23025688_1.fastq.gz	Fastqsanger.gz
	SRR23025688_2.fastq.gz	Fastqsanger.gz
	SRR23025689_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 20

Similar Datasets

Insm1 regulates the expression of thymic tissue-restricted antigens and the immune tolerance [CUT&Tag]

Project description:Insm1, encoding a zinc finger protein, is expressed specifically in neuroendocrine cells. Recent study showed the first evidence of Insm1 expression in medullary thymic epithelial cells (mTEC). Here we investigated the expression and function of Insm1 in mTEC. Mutation of Insm1 resulted in decreased proportion of mTEChi although normal development of other types of thymic cells. We detected altered expression of a subset of tissue-restricted antigens (TRAs) and mild decreased expression of Aire and Fezf2 in Insm1 mutant mTEC. We further showed that Insm1 recognizes a DNA sequence which is similar to the CCCTC-Binding factor (CTCF) binding motif. Mutation of Insm1 altered CTCF binding genome widely and thus the expression of genes. In nude mice transplanted with Insm1 mutant thymus, autoimmune responses were observed in multiple peripheral tissues. In thymus specific Insm1 mutant mice, we detected decreased induction of regulatory T (Treg) cells in the thymus, obvious lymphocytes infiltration and autoimmune antibody reaction in several peripheral tissues. In thymic epithelial cells specific Insm1 overexpression mice, we detected enlarged mTEC proportion and increased expression of mTEC specific genes. Thus, we suggest that Insm1 is a novel regulator of mTEC function and autoimmunity.

2024-01-01 | GSE193926 | GEO

Insm1 regulates the expression of thymic tissue-restricted antigens and the immune tolerance [RNA-seq]

2024-01-01 | GSE193927 | GEO

Insm1 regulates the expression of thymic tissue-restricted antigens and the immune tolerance

Project description:Insm1 regulates the expression of thymic tissue-restricted antigens and the immune tolerance

| PRJNA798413 | ENA

Insm1 regulates the expression of thymic tissue-restricted antigens and the immune tolerance [RNA-seq]

Project description:Insm1 regulates the expression of thymic tissue-restricted antigens and the immune tolerance [RNA-seq]

| PRJNA798415 | ENA

Thymic mimetic cells function beyond self-tolerance [Insm1]

Project description:To examine the Endocrine thymic epithelial cell transcriptome within the mTEC-III compartment we sorted this population from young Insm1 cKO mice and performed bulk RNAseq

2023-08-28 | GSE236073 | GEO

Thymic mimetic cells function beyond self-tolerance [Insm1]

Project description:Thymic mimetic cells function beyond self-tolerance [Insm1]

| PRJNA988544 | ENA

Insm1 regulates the expression of thymic tissue-restricted antigens and the immune tolerance

Project description:This SuperSeries is composed of the SubSeries listed below.

2024-01-01 | GSE193929 | GEO

Expression data from cortical thymic epithelial cells ectopically expressing Aire

2015-08-03 | GSE70326 | GEO

Expression data from cortical thymic epithelial cells ectopically expressing Aire

Project description:Aire in medullary thymic epithelial cells plays an essential role in the negative selection through expression of broad arrays of tissue-restricted antigens. We asked whether Aire could also activate the expression of tissue-restricted antigens in cortical thymic epithelial cells. We established a semi-knockin strain of NOD-background mice expressing Aire under control of the promoter of β5t, a thymoproteasome expressed exclusively in the cortex. We extracted RNA from cortical thymic epithelial cells ectopically expressing Aire and hybridization was performed on Affymetrix. microarrays.

2015-08-03 | E-GEOD-70326 | biostudies-arrayexpress

Jmjd3 is required for tissue-restricted antigens expression in mTECs

Project description:Jmjd3 is required for tissue-restricted antigens expression in mTECs

| PRJNA433532 | ENA