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Transcriptome analysis of piperonylic acid-treated, Meloidogyne graminicola-inoculated rice roots

ABSTRACT: Transcriptome analysis of piperonylic acid-treated, Meloidogyne graminicola-inoculated rice roots

PROVIDER: PRJNA799046 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17681914.fastq.gz	Fastqsanger.gz
	SRR17681915.fastq.gz	Fastqsanger.gz
	SRR17681916.fastq.gz	Fastqsanger.gz
	SRR17681917.fastq.gz	Fastqsanger.gz
	SRR17681918.fastq.gz	Fastqsanger.gz

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Transcriptome analysis of piperonylic acid-treated, Meloidogyne graminicola-inoculated rice roots

Project description:We used mRNA sequencing to study the effects of foliar piperonylic acid treatment on the transcriptome of rice plants (cv. Nipponbare) and infection by M. graminicola to gain insights in the molecular basis of piperonylic acid-induced disease resistance against this nematode

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Project description:In this study we generated small RNA libraries from tissues rice plants infected with Meloidogyne graminicola (Mg)3 days postinoculation as well as from uninfected rice plants. The goal was to study the behaviour of small RNA (miRNA and siRNAs) in response to Mg infection. We combine the expression data gathered from this experiment together with degradome sequencing to predict the targets of differentially expressed miRNAs. Expression was validated using RT-qPCR. The small RNA sequencing datasets were also used with literature annotated siRNA loci for differential expression analysis. DNA methylation data and total RNA sequencing data were used to find siRNAs that were candidates to regulate gene expression through DNA methylation of promoter regions.

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