Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Single-cell RNA-seq of Wild Type and Cd226-/- Thymus Transcriptomes

ABSTRACT: Single-cell RNA-seq of Wild Type and Cd226-/- Thymus Transcriptomes

PROVIDER: PRJNA800037 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR17720890_1.fastq.gz	Fastqsanger.gz
	SRR17720890_2.fastq.gz	Fastqsanger.gz
	SRR17720891_1.fastq.gz	Fastqsanger.gz
	SRR17720891_2.fastq.gz	Fastqsanger.gz

Items per page:

1 - 4 of 4

Similar Datasets

Single-cell RNA-seq of Wild Type and Cd226-/- Thymus Transcriptomes

Project description:Thymi from WT and Cd226 strain knockout mice were digested to obtain single-cell suspensions and then loaded onto microfluidic devices for scRNA-seq. The scRNA-seq libraries were constructed and sequencing outputs were demultiplexed using the bcl2fastq to convert Binary Base Call files into FASTQ format.

2022-01-27 | GSE194270 | GEO

Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and Cd226-/- BMDM Transcriptomes

Project description:Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and Cd226-/- BMDM Transcriptomes

| PRJNA753298 | ENA

Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and Cd226-/- BMDM Transcriptomes

Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare WT-BMDM vs. cd226-BMDM transcriptome profiling (RNA-seq) to microarray and quantitative reverse transcription polymerase chain reaction (qRT–PCR) methods and to evaluate protocols for optimal high-throughput data analysis

2024-08-10 | GSE181758 | GEO

Blocking CD226 regulates ILC2 effector function and alleviates airway hyperreactivity

Project description:Type 2 innate lymphoid cells (ILC2s) play a pivotal role in type 2 asthma. CD226 is a costimulatory molecule involved in various inflammatory diseases. Here, we aimed to investigate CD226 expression and function within human and mouse ILC2s, and to assess the impact of targeting CD226 on ILC2-mediated airway hyperreactivity (AHR). Our findings demonstrated an inducible expression of CD226 in activated ILC2s, enhancing their cytokine secretion and effector functions. Blocking CD226 ameliorates ILC2-dependent AHR in IL-33 and Alternaria Alternata-induced models. Interestingly, CD226 is expressed and inducible in human ILC2s, and its blocking reduces cytokine production. Finally, we showed that peripheral ILC2s in asthmatic patients exhibited elevated CD226 expression compared to healthy controls.Our findings underscore the potential of CD226 as a novel therapeutic target in ILC2s, presenting a promising avenue for ameliorating AHR and allergic asthma.

2024-04-16 | GSE264060 | GEO

Analysis of Wild Type and MFN2-/- Macrophage Transcriptomes

Project description:Analysis of Wild Type and MFN2-/- Macrophage Transcriptomes

| PRJNA715471 | ENA

Transcriptomic analysis of freshly purified human CD226- and CD226+ CD8+ T cell subsets isolated from the peripheral blood of healthy donors.

Project description:We recently found that loss of the activating receptor CD226 (DNAM-1) was a critical mechanism affecting CD8+ T cell responsiveness to TCR stimulation. To better understand the molecular differences between CD226- and CD226+ CD8+ T cells, we decided to perform a global transcriptional analysis of purified naive (Tn, CD62L+CD45RA+), central memory (Tcm, CD62L+CD45RA-) and terminal effector memory (Temra, CD62L-CD45RA+) CD226+ and CD226- CD8+ T cells using next generation RNA sequencing.

2020-10-30 | GSE135291 | GEO

Transcriptomic analysis of resting or activated human CD226- and CD226+ effector memory CD8+ T cell subsets isolated from the peripheral blood of healthy donors.

Project description:We recently found that loss of the activating receptor CD226 (DNAM-1) was a critical mechanism affecting CD8+ T cell responsiveness to TCR stimulation. To better understand the molecular differences between CD226- and CD226+ CD8+ T cells, we decided to perform a global transcriptional analysis of purified CD226+ and CD226- CD8+ effector memory T cells before and after TCR activation using next generation RNA sequencing. We report here the total RNA sequencing of healthy donor resting CD226- and CD226+ CD8+ Tem cells (n=6/group) or activated (ACT, n=4/group) by α-CD2/CD3/CD28 for 24 hrs.

2020-10-30 | GSE133397 | GEO

RNA-seq of wild-type iNKT1 cells from thymus, liver and spleen

Project description:RNA-seq of wild-type iNKT1 cells from thymus, liver and spleen

| PRJNA889806 | ENA

Quantitative analysis of wild type and set2 mutant transcriptomes

Project description:Quantitative analysis of wild type and set2 mutant transcriptomes

| PRJNA805230 | ENA

Analysis of Wild Type and Whsc1-/- small intestine Transcriptomes

Project description:Analysis of Wild Type and Whsc1-/- small intestine Transcriptomes

| PRJNA743911 | ENA