Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Comprehensive identification of RNA transcripts and construction of RNA networks in coronary arterial calcification

ABSTRACT: Comprehensive identification of RNA transcripts and construction of RNA networks in coronary arterial calcification

PROVIDER: PRJNA800089 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

ENPP1 deletion and infantile arterial calcification

Project description:The goal of this study was to identify new mutations in the ENPP1 gene that produce infantile arterial calcification and fetal demise. A stillborn (proband) was diagnosed with infantile arterial calcification. Mutations in the ENPP1 gene account for ~80% of the cases of infantile arterial calcification through loss of function in both alleles (recessive inheritance).

2011-01-26 | GSE26860 | GEO

Integrative DNA, RNA and protein evidence connects TREML4 to coronary artery calcification

2014-06-10 | GSE58150 | GEO

Integrative DNA, RNA and protein evidence connects TREML4 to coronary artery calcification

Project description:Peripheral blood RNA-Seq from human coronary artery calcification cases and controls; Coronary artery calcification (CAC) is a heritable and definitive morphologic marker of atherosclerosis that strongly predicts risk for future cardiovascular events. To search for genes involved in CAC, we used an integrative transcriptomic, genomic, and protein expression strategy using next-generation DNA sequencing in the discovery phase with follow-up studies using traditional molecular biology and histopathology techniques. Eight cases and eight controls (matched for gender, age and ancestry); RNA sequencing of peripheral blood from a discovery set of eight CAC cases and eight matched controls was used to identify dysregulated genes, which were validated using the NanoString® and Affymetrix GeneChip® Human Exon ST Array platforms. The median CAC scores for cases in the screening and validation sets were 1531.5 and 668.5, respectively, while all controls had a score of zero.

2014-06-10 | E-GEOD-58150 | biostudies-arrayexpress

Comprehensive identification of RNA transcripts and construction of RNA networks in coronary arterial calcification

Project description:Here, we report a comprehensive analysis of dysregulated mRNAs, lncRNAs, miRNAs and circRNAs in peripheral blood of CAC patients with high-throughput RNA sequencing. We mapped about 50 million sequence reads per sample to the human genome. GSEA analysis showed that these dysregulated RNAs correlate with several critical biological processes such as RNA modification, and RNA metabolism. RT-qPCR (SYBR Green assays) with more clinical CAC samples were used for the validation of some dysregulated RNAs. We have also constructed the co-expression network between lncRNA and mRNA, and established the circRNA-miRNA-mRNA in CAC based on this data. This study provides valuable insights to understand the RNA involvement and regulation in CAC, assisting future CAC investigations.

2022-07-01 | GSE194304 | GEO

Coronary arterial development is regulated by a Dll4-Jag1-EphrinB2 signaling cascade

Project description:Coronaries are essential for myocardial growth and heart function. Notch is crucial for mouse embryonic angiogenesis, but its role in coronary development remains uncertain. We show Jag1, Dll4 and activated Notch1 receptor expression in sinus venosus (SV) endocardium. Endocardial Jag1 removal blocks SV capillary sprouting, while Dll4 inactivation stimulates excessive capillary growth, suggesting that ligand antagonism regulates coronary primary plexus formation. Later endothelial ligand removal, or forced expression of Dll4 or the glycosyltransferase MFng, blocks coronary plexus remodeling, arterial differentiation, and perivascular cell maturation. Endocardial deletion of Efnb2 phenocopies the coronary arterial defects of Notch mutants. Angiogenic rescue experiments in ventricular explants, or in primary human endothelial cells, indicate that EphrinB2 is a critical effector of antagonistic Dll4 and Jag1 functions in arterial morphogenesis. Thus, coronary arterial precursors are specified in the SV prior to primary coronary plexus formation and subsequent arterial differentiation depends on a Dll4-Jag1-EphrinB2 signaling cascade.

2019-12-03 | GSE110614 | GEO

MicroRNA expression profiling for coronary artery calcification in Indian chronic kidney disease patients

Project description:Our study investigates the expression profilling of miRNAs in the pathogenesis of coronary artery calcification in chronic kidney disease patients and identify alter miRNA gene expression which will gives a unique platform for new drug discovery

2017-12-01 | GSE89699 | GEO

RNA-seq data of aortas with atherosclerotic or medial calcification

Project description:Vascular calcification is the ectopic deposition of calcium hydroxyapatite minerals in arterial wall. However, the underlying molecular mechanisms regulating vascular calcification remain incompletely understood. In this study, we applied RNA sequencing to explore the mechanism of vascular calcificaiton in both medial and atherosclerotic vascular calcification models.

2021-02-23 | GSE159832 | GEO

LncRNA expression profile and target gene prediction of calcification in human aortic smooth muscle cells induced by DPP4

Project description:Arterial media calcification caused by diabetes is an important cause of vascular calcification. Dipeptidyl peptidase-4 (DPP4) is associated with diabetic arterial media calcification. At the same time, long non-coding RNA(lncRNA) is closely related to the evolution of a variety of cardiovascular diseases, but the involvement of lncRNA in vascular calcification induced by DPP4 has not been reported in details. In this study, we established a model of human aortic smooth muscle cells (HASMCs) calcification induced by DPP4. There was a significant difference in the expression of lncRNAs and mRNAs between normal and calcified vascular smooth muscle cells detected by gene chip technology. Based on the results of microarray detection, we found that lncRNA may be involved in vascular calcification induced by DPP4 through regulating target genes.

2021-12-03 | GSE189923 | GEO

Circulating miRNA profile and coronary calcification in asymptomatic patients with familial hypercholesterolemia.

Project description:Circulating miRNA profile and coronary calcification in asymptomatic patients with familial hypercholesterolemia.

| PRJNA737428 | ENA

NHLBI TOPMed: Diabetes Heart Study African American Coronary Artery Calcification (AA CAC)

Project description:NHLBI TOPMed: Diabetes Heart Study African American Coronary Artery Calcification (AA CAC)

| PRJNA394787 | ENA