Project description:We revealed that the MPLSST neurons are excitatory neurons releasing neurotransmitter glutamate,and reveal that more than half of these neurons corelease neuropeptides SST and PTH2
Project description:We report bulk RNAseq data obtained from the Anterior Cingulate Cortex (ACC) microdissected from Untrained (n=5) and Trained (n=6) wild-type mice for an instrumental task, the lever-press task for food reward.
Project description:CUT&RUN for MEF2C in mature PV+ and SST+ cortical interneurons to characterize the differential usage of this transcription factor by these populations.
Project description:In the cerebellum, the excitatory neurons of the three cerebellar nuclei (eCN) form the primary output for the circuit. The medial eCN (eCNm) were recently divided into molecularly defined subdomains in the adult, however how they are established during development is not known. We define molecular subdomains of the eCNm using scRNA-seq and spatial expression analysis during embryogenesis. Our analysis shows that the eCNm are transcriptionally divergent from the rest of the eCN by E14.5. We previously showed that conditional loss of the homeobox genesEn1andEn2leads to death of a subset of embryonic eCNm. We demonstrate that loss ofEn1/2in embryonic eCNm results in cell death of a specific posterior eCNm molecular subdomain and loss of TBR2 expression in an anterior subdomain, as well as reduced synaptic gene expression. We further reveal a similar function for EN1/2 in mediating TBR2 expression, neuron differentiation and survival in the two other cerebellar excitatory neuron types. Thus, our work defines embryonic eCNm molecular diversity and reveals conserved roles for EN1/2 in the cerebellar excitatory neuron lineage.