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MEG3 activates necroptosis in human neuron xenografts modeling Alzheimer’s disease II

ABSTRACT: MEG3 activates necroptosis in human neuron xenografts modeling Alzheimer’s disease II

PROVIDER: PRJNA800741 | ENA |

REPOSITORIES: ENA

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MEG3 activates necroptosis in human neuron xenografts modeling Alzheimer’s disease

Project description:Neuronal cell loss is a defining feature of Alzheimer’s disease (AD), but the underlying mechanisms remain unclear. We xenografted human or mouse neurons into the brain of a mouse model of AD. Only human neurons displayed tangles, Gallyas silver staining, granulovacuolar neurodegeneration (GVD), phosphorylated tau blood biomarkers, and considerable neuronal cell loss. The long noncoding RNA MEG3 was strongly up-regulated in human neurons. This neuron-specific long noncoding RNA is also up-regulated in AD patients. MEG3 expression alone was sufficient to induce necroptosis in human neurons in vitro. Down-regulation of MEG3 and inhibition of necroptosis using pharmacological or genetic manipulation of receptor-interacting protein kinase 1 (RIPK1), RIPK3, or mixed lineage kinase domain-like protein (MLKL) rescued neuronal cell loss in xenografted human neurons. This model suggests potential therapeutic approaches for AD and reveals a human-specific vulnerability to AD.

2023-09-15 | GSE195455 | GEO

MEG3 activates necroptosis in human neuron xenografts modeling Alzheimer’s disease

Project description:MEG3 activates necroptosis in human neuron xenografts modeling Alzheimer’s disease

| PRJNA800742 | ENA

MEG3 activates necroptosis in human neuron xenografts modeling Alzheimer’s disease II

2023-09-15 | GSE195457 | GEO

MEG3 activates necroptosis in human neuron xenografts modeling Alzheimer’s disease I

Project description:MEG3 activates necroptosis in human neuron xenografts modeling Alzheimer’s disease I

| PRJNA800730 | ENA

MEG3 activates necroptosis in human neuron xenografts modeling Alzheimer’s disease I

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-09-15 | GSE195458 | GEO

MEG3 activates necroptosis in human neuron xenografts modeling Alzheimer's disease.

Project description:Neuronal cell loss is a defining feature of Alzheimer's disease (AD), but the underlying mechanisms remain unclear. We xenografted human or mouse neurons into the brain of a mouse model of AD. Only human neurons displayed tangles, Gallyas silver staining, granulovacuolar neurodegeneration (GVD), phosphorylated tau blood biomarkers, and considerable neuronal cell loss. The long noncoding RNA MEG3 was strongly up-regulated in human neurons. This neuron-specific long noncoding RNA is also up-regulated in AD patients. MEG3 expression alone was sufficient to induce necroptosis in human neurons in vitro. Down-regulation of MEG3 and inhibition of necroptosis using pharmacological or genetic manipulation of receptor-interacting protein kinase 1 (RIPK1), RIPK3, or mixed lineage kinase domain-like protein (MLKL) rescued neuronal cell loss in xenografted human neurons. This model suggests potential therapeutic approaches for AD and reveals a human-specific vulnerability to AD.

| S-EPMC7615236 | biostudies-literature

ChIP-seq in MEG3+ and MEG3- hIPSCs

Project description:In this experiment we sought to identify region with differential PRC2, JARID2, and H3K27me3 occupancy in human induced pluripotent stem cell lines with silenced or expressed MEG3 locus to indirectly determine the effect of this ncRNA on PRC2 function. 8 hIPSC lines, 5 MEG3+ and 3 MEG3-; ChIP-seq for EZH2, JARID2, and H3K27me3. Some done in replicates.

2013-12-25 | E-GEOD-48516 | biostudies-arrayexpress

overexpression/knockdown MEG3 in C2C12 cell lines

Project description:MEG3 was known as a growth suppressor in tumor cells by activating p53. Besides, MEG3 could regulate transforming growth factor-β (TGF-β) signaling pathway, which is the key regulator of skeletal myogenesis and can enhance the proliferation of myogenic cells. Previous study also showed MEG3 was highly expressed in the paraxial mesoderm and probably regulated muscle development. To investigate the potential function of MEG3 in muscle development, we detected the expression levels of protein-coding genes after MEG3 over-expression or knockdown in C2C12 cell line using microarrays.

2018-09-28 | GSE73524 | GEO

Meg3 regulated genes in pancreatic beta cells

Project description:Meg3 is a long non-coding RNA. It's target genes are unknown. The mouse pancreatic beta cell line MIN6-4N was used to assess the expression of genes upon stable Meg3 overexpression Stable cell lines were isolated that have integated pcDNA3.1 or pcDNA3.1-mMeg3.The cells were processed for RNA isolation. The level of Meg3 expression was assessed by RT-PCR. RNA preps were used for microarray analysis.

2015-05-21 | E-GEOD-57716 | biostudies-arrayexpress

ChIP-seq in MRC5 hIPSCs overexpressing MEG3 RNA.

Project description:This experiment was designed to detect differences in PRC2 occupancy in a human induced pluripotent cell line (MRC5) naturally deficient in MEG3 RNA after lentiviral overexpression of MEG3. MRC5 were transduced with lentiviruses for the overexpression of MEG3 lncRNA or GFP as a control. ChIP-seq was performed with EZH2 and JARID2 antibodies.

2013-12-25 | E-GEOD-48515 | biostudies-arrayexpress

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