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Characterization and function of transcriptional elongation during zygotic genome activation in mouse embryos [RNA-seq]

ABSTRACT: Characterization and function of transcriptional elongation during zygotic genome activation in mouse embryos [RNA-seq]

PROVIDER: PRJNA802262 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17839000_1.fastq.gz	Fastqsanger.gz
	SRR17839000_2.fastq.gz	Fastqsanger.gz
	SRR17839001_1.fastq.gz	Fastqsanger.gz
	SRR17839001_2.fastq.gz	Fastqsanger.gz
	SRR17839002_1.fastq.gz	Fastqsanger.gz

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Characterization and function of transcriptional elongation during zygotic genome activation in mouse embryos [RNA-seq]

Project description:The work associated to these datasets focuses on the investigation of transcriptional regulation during the earliest stages of mammalian embryogenesis. In particular, ChILseq and RNAseq datasets were generated to study the process of zygotic genome activation in mouse embryos. After fertilisation, the mouse embryo activates its own genome for the first time in a process known as zygotic genome activation, which occurs primarily on two main waves. The first wave occurs in zygotes, and the second one at the late 2-cell stage. To understand how the RNA Polymerase II functions during these waves, we have implemented a ChILseq approach to generate genome-wide maps of Ser5 and Ser2 phosphorylated forms of RNA Polymerase II in zygotes, 2- and 8-cell stage embryos. In addition, we have investigated how elongation factors, such as NELF and DSIF regulate major ZGA by performing single embryo RNAseq using SMART-seq2 in 2-cell stage embryos upon depletion of Spt5, NELFE and upon chemical inhibition of Cdk9.

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Project description:Characterization and function of transcriptional elongation during zygotic genome activation in mouse embryos [ChIL-seq]

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Project description:A conserved event of the maternal-to-zygotic transition (MZT) in animal embryos is the elimination of a subset of the maternal transcripts that accumulated during oogenesis. In invertebrates and lower vertebrates, a maternally encoded mRNA decay pathway (M-decay) acts before zygotic genome activation (ZGA) while a second pathway, which requires zygotic transcription, subsequently clears additional mRNAs (Z-decay). To date it has not been clear whether a Z-decay pathway is present in mammals. Here, we identify mouse and human maternal transcripts that are degraded after ZGA and show that inhibition of de novo transcription stabilizes these mRNAs in mouse and human embryos. We show that YAP1-TEAD4 transcription factor-mediated transcription is essential for Z-decay in mouse embryos and that TEAD4-triggered zygotic Tut4/7 expression and mRNA 3ʹ-oligouridylation direct Z-decay.

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