Project description:Our study represents the first detailed analysis of thyroid hormone binding to the genome in the tail of wild-type and TRα (-/-) tadpoles, with biological replicates, generated by ChIP-seq technology. The optimized data analysis workflows reported here should provide a framework for comparative investigations of TR binding profiles.
Project description:Premetamorphic Xenopus laevis tadpole tail respond to thyroid hormone by resorption. The goal of this experiment is to identify the genes involved in the TH-induced resorption tadpole tail and compare it to TH-induced proliferation and differentiation program in tadpole limb and brain. Xenopus tadpoles (NF54) were treated with 100 nM T3 in 0.1 x MMR for another 24h and 48h or without T3 for 48h (control group). NF 61 tadpoles were in 0.1 X MMR till they reached NF stage 62. The tails were dissected after the experiment. Keywords: development or differentiation design,organism part comparison design,reference design,replicate design,time series design
Project description:Hypothyroids wild type and TRbeta -/- mice were treated with T3 and livers were collected and analysed using a microarray with 6500 cDNA probes. Expression ratios were calulated between 1) hypothyroids WT and TRbeta-/- mice 2) hypothyroid WT and hypothyroid TR beta-/- mice treated with T3 and 3) hypothyroid TRbeta-/- and hypothyroid TRbeta-/- treated with T3. Keywords: other
Project description:Our study represents the first detailed analysis of thyroid hormone binding to the genome in the hindlimb of wild-type and TRα (-/-) tadpoles, with biological replicates, generated by ChIP-seq technology. The optimized data analysis workflows reported here should provide a framework for comparative investigations of TR binding profiles.
Project description:Analysis of T3 response in KCL034, iKCL004 and BJ cells at gene expression level. Results provide information of the T3 response in different lines.
Project description:To investigate what genes/pathways that specific induced by T3/TRs which inhibited tail regeneration. In addition, to detect the gene regulation program that important for regeneration were altered by T3 through TRs during the initiation period of tail regeneration