Ontology highlight
ABSTRACT:
PROVIDER: PRJNA802905 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR17867519_1.fastq.gz | Fastqsanger.gz | |||
| SRR17867519_2.fastq.gz | Fastqsanger.gz | |||
| SRR17867520_1.fastq.gz | Fastqsanger.gz | |||
| SRR17867520_2.fastq.gz | Fastqsanger.gz | |||
| SRR17867521_1.fastq.gz | Fastqsanger.gz |
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Project description:Tendon is a highly organized, dense connective tissue that has been demonstrated to have very little turnover. In spite of the low turnover, tendon can grow in response to loading, which may take place primarily at the periphery. Tendon injuries and recurrence of injuries are common in both human and animal in sports. It is unclear why some areas of the tendon are more susceptible to such injury and whether this is due to intrinsic regional differences in extracellular matrix (ECM) production or tissue turnover. This study aimed to compare populations of tenocytes derived from the tendon core and periphery. Tenocytes were isolated from equine superficial digital flexor tendons (SDFT), and the proliferation capacity was determined. ECM production was characterized by immuno- and histological staining and by liquid chromatography-mass spectrometry-based proteomics. Core and periphery SDFT cultures exhibited comparable proliferation rates and had very similar proteome profiles, but showed biological variation in collagen type I deposition. In conclusion, the intrinsic properties of tenocytes from different regions of the tendon are very similar and other factors in the tissue may contribute to how specific areas respond to loading or injury.
2020-08-24 | PXD019974 | Pride
Project description:Tendon is a highly aligned connective tissue, in which the macro-structure consists of collagen-rich fascicles surrounded by interfascicular matrix (IFM). In a series of recent studies in equine tissue, we have demonstrated specialisation of tendon composition, structure and mechanics to achieve the tendon’s functional requirements, specifically reporting extensive specialisation of the IFM region in the energy storing superficial digital flexor tendon. We have also demonstrated loss of functional specialisms with ageing, leading to a hypothesised new paradigm for tendinopathy, focused on the importance of the IFM. However, to date, there have been no studies focused on structure-function specialisation or the IFM in functionally distinct human tendons. Here, we compare the positional anterior tibialis tendon and energy storing Achilles tendon, performing a detailed analysis of the composition and mechanical properties of both fascicle and IFM regions, to test the hypothesis that the IFM in the energy storing Achilles tendon has specialised composition and mechanical properties, and that these specialisations are lost with ageing. We demonstrate that the IFM is specialised in the energy storing Achilles tendon, with greater elasticity and fatigue resistance than in the positional anterior tibialis tendon. While there were few age-related alterations in mechanics, we did identify age-related alterations in the IFM proteome of the Achilles tendon specifically, which is predicted to be regulated by TGF-beta signalling and may be responsible for the trend towards decreased fatigue resistance observed in the Achilles IFM with ageing.
2020-11-11 | PXD018212 | Pride
Project description:Purpose: The goal of this study is to compare transcriptional profiles of flexor tendon healing in wild-type (WT, C57Bl/6J) to superhealer (MRL/MpJ) miceto gain insights in the biological drivers of the tendon injury response between the C57 and MRL mice. Methods: RNA was isolated from patially lacerated or uninjured flexor tendon 7 days post-injury. Results: Transcriptional analysis of biological drivers showed positive enrichment of TGFB1 in both C57 and MRL healing tendons. only MRL tendons exhibited downstream transcriptional effects of cell cycle regulatory genes, with negative enrichment of the cell senescence-related regulators, compared to the positively-enriched inflammatory and ECM organization pathways in the C57 tendons. Conclusions: There is altered TGFB1 regulated inflammatory, fibrosis, and cell cycle pathways in flexor tendon repair.
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Project description:We report RNA sequencing data from the Achilles, patellar, supraspinatus, and forepaw flexor tendons of adult male rats in the Sprague-Dawley
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Project description:We report RNA sequencing data from the Achilles, patellar, supraspinatus, and forepaw flexor tendons of adult male mice in the C57Bl/6 background.
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Project description:This dataset is comprised of RNA samples from 11 horses of various ages and sex that comprise the following tissues: Adipose Tissue, Adrenal Cortex, Adrenal Medulla, Aorta, Articular Cartilage (foal), Bladder, Bone, Bone Marrow, Cecum, Cerebrum, Cornea, Embryo (whole embryo, 34d), Endometrium (preg. day 16), Endometrium (preg. day 50), Epididymus, Hoof (germinal epithelium), Kidney, Large Intestine, Liver, Lung Lymph Node, Lymphocytes (activated), Muscle (cardiac), Muscle (skeletal, tongue), Muscle (skeletal), Muscle (smooth), Ovary, Pancreas, Pituitary (anterior), Pituitary (posterior), Placental Villous, Retina, Salivary Gland, Skin (full thickness), Spinal Cord, Spinal Root Ganglia, Spleen (red pulp), Spleen (white pulp), Stomach, Synovial Membrane, Tendon (superficial digital flexor, foal), Testes, Thymus, and Vena Cava. This study was carried out in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. Tissue samples for total RNA isolation were collected under a protocol (00814A2004) reviewed and approved by the Office of Research Integrity, Institutional Animal Care and Use Committee, at the University of Kentucky (PHS Assurance Number: A3336-01). Two micrograms of mRNA from each tissue sample was used to generate the final RNA pool. The pooled sample was Illumina and 454 sequenced with the aim to improve equine gene structural annotation.
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Project description:Little is understood about the roles of tendon cells during flexor tendon healing. To better understand tendon cell functions, the Scx-Cre mouse was crossed to the DTR mouse model to facilitate scleraxis lineage cell depletion prior to acute flexor tendon injury and repair. WT (cre-) and experimental (cre+) mice underwent complete transection and repair of the flexor digitorum longus tendon. Repaired tendons were harvested at 14 and 28 days post-repair for bulk RNA-Seq analysis to examine possible mechanisms driving differential healing due to Scx lineage cell depletion.
2021-02-08 | GSE156157 | GEO