Project description:HEK293T cells were transfected with the Rbp1-amr or slow (R729H-amr) Î±-amanitin resistant subunit of RNA Pol II and selected with Î±-amanitin 24 hours after transfection for additional 24 hours. Total RNA was extracted and global changes in gene expression were determined using microarray chips. MiRNAs are transcribed by RNA pol II but the transcriptional features influencing their synthesis are poorly defined. Here we report that a TATA-box in miRNA and a subset of protein-coding genes is associated with increased sensitivity to a slow rate of transcription elongation. We also show that promoters driven by TATA-box or NF-ÎºB elicit high transcription re-initiation rate, but paradoxically lower levels of miRNA. Interestingly, miRNA synthesis was converted to a more productive mode by decreasing initiation rate, but less productive when the re-initiation rate increased. This phenomenon was found to be associated with a delay in miR-146a induction by NF-ÎºB. We also demonstrate that miRNAs are remarkably strong pause sites. Our findings suggest that lower efficiency of miRNA synthesis directed by the TATA-box or NF-ÎºB is a consequence of frequent transcription initiation that lead to Pol II crowding at pause sites, thereby increasing the chance of collision and premature termination. These findings highlight the importance of the transcription initiation mechanism for miRNA synthesis, and have implications for TATA-box promoters in general. HEK293T cells were transfected with plasmids directing the expression of Î±-amanitin-resistant variants of Pol II (Rpb1-amr and R749H-amr). Î±-amanitin was added and RNA was prepared 24 and 48 h later, respectively. The data provided is from 3 Rpb1-amr vs 3 R749H-amr (6 samples).

2013-07-01 | E-GEOD-48163 | biostudies-arrayexpress

AMR target-enriched metagenomic sequence reads from neonatal dairy calf feces

Project description:AMR target-enriched metagenomic sequence reads from neonatal dairy calf feces Raw sequence reads

| PRJNA897887 | ENA

AMR in AD

Project description:AMR in AD

| PRJEB51244 | ENA

FWD-AMR

Project description:sequences for the project FWD-AMR

| PRJEB57330 | ENA

VTEC-AMR

Project description:Verotoxin-producing E.coli genomes with AMR data

| PRJEB21880 | ENA

Expression data from C. elegans exposed to pristine and aged ZnO manufactured nanoparticles

Project description:Caenorhabditis elegans were exposed at EC30 for 48 hr at L1 stage to ZnSO4, pristine ZnO-MNPs, phosphate aged pZnO-MNPs and sulfidized sZnO-MNPs Whole genome whole trascript microarrays were used to examine and compare global gene expression profiles among treatments

2019-02-22 | GSE114881 | GEO

Gene expression profiling of MRC-5 cells treated with 100 ug/mL regular-length pristine SWCNTs for 72 h

Project description:A total of 30 genes were up-regulated and 19 genes were down-regulated > 1.5-fold following 100 ug/mL regular-length pristine SWCNTs treatment

2016-11-23 | GSE89375 | GEO

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