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Ricinus communis cultivar:ZB306

ABSTRACT: RNA-seq for castor bean seedling under heat stress

PROVIDER: PRJNA803603 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17894238_1.fastq.gz	Fastqsanger.gz
	SRR17894238_2.fastq.gz	Fastqsanger.gz
	SRR17894239_1.fastq.gz	Fastqsanger.gz
	SRR17894239_2.fastq.gz	Fastqsanger.gz
	SRR17894240_1.fastq.gz	Fastqsanger.gz

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Physiological, Biochemical, and Transcriptomic Analyses Reveal the Response to Oxidative Stress Under Drought Stress in Castor (Ricinus communis L.)

Project description:Castor is an important industrial raw material. Drought leads to delayed development and reduced yield of castor plants. However, the molecular mechanism of the response of castor plants to drought stress has not been determined. In this study, physiological, biochemical, and RNA-seq analyses were conducted on castor roots under PEG-6000 stress for 3, 7, and 4 d (11 d) of rehydration. The activities of GR, APX, SOD, and CAT in the roots of the castor plants under PEG-6000 stress increased at 3 d and decreased at 7 d, while the activity of POD continued to increase. A total of 2926, 1507, and 111 DEGs were identified in castor roots stressed with PEG-6000 for 3 d and 7 d and rehydrated for 4 d (11 d), respectively. GO analysis revealed that antioxidant activity was significantly enriched under PEG stress. In addition, KEGG enrichment analysis revealed that the DEGs were significantly involved in glutathione metabolism, fatty acid metabolism, and plant hormone signal transduction. KEGG combined with WGCNA was used to screen two drought stress-related genes, PP2C39 and GA2ox4. We propose an oxidative stress model for castor plants under drought stress based on GO terms, KEGG pathway, and WGNA analyses. This study identified high-quality genes for the genetic and molecular breeding of drought-resistant castor beans.

2024-10-23 | GSE252706 | GEO

Ricinus communis cultivar:IAC Guarani

Project description:Analysis of Castor bean response to drought stress

| PRJNA401329 | ENA

Quantitative proteomic analysis of Ricinus communis during early seed imbibition provided novel insights into cold stress response

Project description:Early planting is one of the strategies used to increase grain yield in temperate regions. However, poor cold tolerance in castor inhibits seed germination, resulting in lower seedling emergence and biomass. To date, proteomics studies have identified diverse proteins associated with germinating or developing castor seeds; however, the regulators involved in early imbibed castor seeds under cold stress are rare. Here, the elite castor variety Tongbi 5 was used to identify the differential abundance protein species (DAPS) between cold stress (4 °C) and control conditions (30 °C ) imbibed seeds. As a result, 127 proteins were identified to be differentially accumulated according to iTRAQ strategy. Bioinformatics analyses revealed that 84 DAPS were annotated in 24 GO functional groups, 70 DAPS were classified into 16 COG categories, and 56 DAPS were involved in 16 KEGG pathways. Based on physiological index assays and iTRAQ results, this study provided some invaluable insights into the cold stress responses of early imbibed castor seeds.

2018-06-05 | PXD010043 |

Project description:stress response of castor bean seedling

| PRJNA872766 | ENA

Deciphering Molecular Mechanism of Intermediate Secondary Growth and Internode Elongation of Castor Bean (Ricinus communis L.) by combined analysis of transcriptome and metabolome

Project description:The length of internodes is critical in determining the height of the castor plant (Ricinus communis L.), and is closely associated with internode elongation. However, the exact mechanisms underlying internode elongation, particularly in the main stem of the castor plant, remain uncertain. To investigate further, we conducted a study using the dwarf castor variety 071113, comparing it with the homologous high-stalk Zhuansihao as a control. Our research included cytological observation, physiological measurement, transcriptome sequencing, and metabolic determination. By integrating these findings, we discovered that the dwarf 071113 undergoes earlier main stem lignification development and has a more active lignin synthesis pathway in internode intermediate development. The plant hormone IAA also plays a role in this process. Furthermore, potential enzymes and regulators have been identified, including the auxin influx carrier AUX1 LAX, auxin response protein IAA13, ARF3, auxin-responsive protein SAUR50, peroxidase, and EXPs that regulate cell cycle, cell wall synthesis, as well as growth and development, were also. Based on these findings, we developed a model for castor internode elongation and gained a better understanding of the dwarfing mechanism of the 071113 variety. Our work lays a theoretical foundation for the future breeding of dwarf castor varieties.

2024-01-31 | GSE242985 | GEO

Ricinus communis

Project description:global studies of sucrose signaling on caster bean seeds

| PRJNA393233 | ENA

Tissue-Specific Differences in Metabolites and Transcripts Contribute to the Heterogeneity of Ricinoleic Acid Accumulation in Ricinus communis L. (castor) Seeds

Project description:To examine the differential gene expression of genes invovled in hydroxy fatty acid accumulation within the endosperm and embryo tissues of castor seeds

2019-04-11 | GSE119624 | GEO

Proteomes of Floral and Extrafloral Nectar from the Castor Plant (Ricinus communis)

Project description:Label-free quantitative proteome analysis of extrafloral (EFN) and floral nectar (FN) from castor (Ricinus communis) plants.

2018-10-19 | PXD009104 | Pride

Endogenous small-noncoding RNAs and their roles in cold response and stress acclimation in Cassava

Project description:Small noncoding RNA (sncRNA), including microRNAs (miRNAs) and endogenous small-interfering RNAs (endo-siRNAs) are key gene regulators in eukaryotes, playing critical roles in plant development and stress tolerance. Trans-acting siRNAs (ta-siRNAs), which are secondary siRNAs triggered by miRNAs, and siRNAs from natural antisense transcripts (nat-siRNAs) are two well-studied classes of endo-siRNAs. In order to understand sncRNAsM-bM-^@M-^Y roles in plant cold response and stress acclimation, we studied miRNAs and endo-siRNAs in Cassava (Manihot esculenta), a major source of food for the world populations in tropical regions. Combining Next-Generation sequencing and computational and experimental analyses, we profiled and characterized sncRNA species and mRNA genes from the plants that experienced severe and moderate cold stresses, that underwent further severe cold stress after cold acclimation at moderate stress, and that grew under the normal condition. We also included Castor bean (Ricinus communis) to understand conservation of sncRNAs. In addition to known miRNAs, we identified dozens of novel miRNAs as well as ta-siRNA-yielding and nat-siRNA-yielding loci in Cassava and Castor bean, respectively. Among the expressed sncRNAs, many sncRNAs were differentially expressed under cold stresses. Our study provided the results on gene regulation by sncRNAs in cold acclimation of Euphorbiaceous plants and the role of sncRNA-mediated pathways affected by cold stress and stress acclimation in Cassava. Examination of small RNA populations in Cassava cultivar SC124 under the normal condition (NC), gradual cold acclimation (CA), cold shock (CS) and stress acclimation Cold stress after cold acclimation (CCA).

2014-07-05 | E-GEOD-52177 | biostudies-arrayexpress

Endogenous small-noncoding RNAs and their roles in cold response and stress acclimation in Cassava

Project description:Small noncoding RNA (sncRNA), including microRNAs (miRNAs) and endogenous small-interfering RNAs (endo-siRNAs) are key gene regulators in eukaryotes, playing critical roles in plant development and stress tolerance. Trans-acting siRNAs (ta-siRNAs), which are secondary siRNAs triggered by miRNAs, and siRNAs from natural antisense transcripts (nat-siRNAs) are two well-studied classes of endo-siRNAs. In order to understand sncRNAs’ roles in plant cold response and stress acclimation, we studied miRNAs and endo-siRNAs in Cassava (Manihot esculenta), a major source of food for the world populations in tropical regions. Combining Next-Generation sequencing and computational and experimental analyses, we profiled and characterized sncRNA species and mRNA genes from the plants that experienced severe and moderate cold stresses, that underwent further severe cold stress after cold acclimation at moderate stress, and that grew under the normal condition. We also included Castor bean (Ricinus communis) to understand conservation of sncRNAs. In addition to known miRNAs, we identified dozens of novel miRNAs as well as ta-siRNA-yielding and nat-siRNA-yielding loci in Cassava and Castor bean, respectively. Among the expressed sncRNAs, many sncRNAs were differentially expressed under cold stresses. Our study provided the results on gene regulation by sncRNAs in cold acclimation of Euphorbiaceous plants and the role of sncRNA-mediated pathways affected by cold stress and stress acclimation in Cassava.

2014-07-05 | GSE52177 | GEO

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