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CUT&Tag sequencing in PDAC cells with Setd2-KO and Setd2-WT sorted from orthotopic PDAC tumor

ABSTRACT: CUT&Tag sequencing in PDAC cells with Setd2-KO and Setd2-WT sorted from orthotopic PDAC tumor

PROVIDER: PRJNA804002 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17906251_1.fastq.gz	Fastqsanger.gz
	SRR17906251_2.fastq.gz	Fastqsanger.gz
	SRR17906252_1.fastq.gz	Fastqsanger.gz
	SRR17906252_2.fastq.gz	Fastqsanger.gz
	SRR17906253_1.fastq.gz	Fastqsanger.gz

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CUT&Tag sequencing in PDAC cells with Setd2-KO and Setd2-WT sorted from orthotopic PDAC tumor

Project description:To gain mechanistic insight into how Setd2 loss reprogramming tumor cell metabolism, we performed CUT&Tag sequencing in PDAC cells with Setd2-KO and Setd2-WT sorted from orthotopic PDAC tumor. Setd2-WT and -KO murine PDAC cells were sorted with DAPI-CD45.2-Pdpn-Epcam+ or cultured, and freshly collected as soon as possible. Hyperactive pA-Tn5 Transposase for CUT&Tag kit from Vazyme (TD901), and antibodies against H3K36me3, H3K27Ac and H3K27me3 were employed. Trueprep index kit v2 and v3 for illumina were used to establish DNA library.

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Bulk RNA-seq analysis of PDAC tumor Cells

Project description:Orthotopic PDAC tumor were established with KPC and KPSC cells, respectively. After about 10-14 days, PDAC cells with Setd2-KO and Setd2-WT were sorted from orthotopic PDAC tumor by DPAI-CD45.2-Pdpn-Epcam+, and used for RNA preparation.

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Project description:CUT&Tag analysis of H3K36me3 and H3K27me3, in Setd2-WT and -KO murine PDAC cell line

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Role of Setd2 in regulatory T cells

Project description:This SuperSeries is composed of the SubSeries listed below. To determine the molecular regulation of Tregs by Setd2, Spleen or large intestine Tregs (CD3+CD4+Foxp3-YFP+) were purified from littermate Foxp3Cre-YFPSetd2f/+ and Foxp3Cre-YFPSetd2f/f mice. Setd2-deficient and control Tregs were subjected to RNA-seq, RNA Polymerase II CUT&Tag or H3K27ac CUT&Tag analyses.

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Role of Setd2 in regulatory T cells [H3K27ac CUT&Tag]

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