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PRC2 shields naïve human pluripotent cells from trophectoderm and mesoderm differentiation [scRNA-seq]

ABSTRACT: PRC2 shields naïve human pluripotent cells from trophectoderm and mesoderm differentiation [scRNA-seq]

PROVIDER: PRJNA804150 | ENA |

REPOSITORIES: ENA

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PRC2 shields naïve human pluripotent cells from trophectoderm and mesoderm differentiation [scRNA-seq]

Project description:Single-cell RNA-seq reveals that Polycomb repressive complex 2 (PRC2) maintains naïve pluripotency and restricts an intrinsic capacity of pre-implantation pluripotent stem cells to give rise to trophectoderm and mesoderm lineages. Inhibition of PRC2 forces naïve hESC into an ‘activated’ state through which differentiation into either trophectoderm or mesoderm lineages is triggered. This trajectory is distinct from embryonic lineage specification out of the post-implantation pluripotent state, hence PRC2-mediated repression provides a highly adaptive mechanism to restrict lineage potential during early human development.

2022-03-14 | GSE196280 | GEO

PRC2 shields naive pluripotent cells from trophectoderm and mesoderm differentiation.

Project description:PRC2 shields naive pluripotent cells from trophectoderm and mesoderm differentiation.

| PRJNA751099 | ENA

PRC2 shields naive pluripotent cells from trophectoderm and mesoderm differentiation [ChIP-seq]

Project description:The first lineage choice made in human embryo development separates trophectoderm from the inner cell mass, which proceeds to form the pluripotent epiblast and primitive endoderm. Trophectoderm on the other hand gives rise to the placenta. Naïve pluripotent stem cells are derived from the pluripotent epiblast of the blastocyst and offer possibilities to explore how lineage integrity is maintained. Here, we discover that Polycomb repressive complex 2 (PRC2) restricts an intrinsic capacity of naïve pluripotent stem cells to give rise to trophectoderm. Through quantitative epigenome profiling, we find that broad histone H3 lysine 27 trimethylation (H3K27me3) hypermethylation is a common feature of naïve pluripotency across species. We define a previously unappreciated, naïve-specific set of bivalent promoters, featuring PRC2-mediated H3K27me3 concomitant with H3K4me3. Naïve bivalency maintains key trophectoderm transcription factors in a transcriptionally poised state that is resolved to an active state upon depletion of H3K27me3 via inhibition of the enzymatic subunits of PRC2, EZH1/2. Conversely, primed human embryonic stem cells cannot be driven towards trophectoderm development via PRC2 inhibition. While naïve and primed hESCs share the majority of bivalent promoters, PRC2 contributes to the repression of largely non-overlapping subsets of these promoters in each state, hence H3K27me3-mediated repression provides a highly adaptive mechanism to restrict lineage potential during early human development.

2021-08-23 | GSE181241 | GEO

PRC2 shields naive pluripotent cells from trophectoderm and mesoderm differentiation [RNA-seq]

2021-08-23 | GSE181242 | GEO

PRC2 shields naive pluripotent cells from trophectoderm and mesoderm differentiation [RNA-seq]

Project description:PRC2 shields naive pluripotent cells from trophectoderm and mesoderm differentiation [RNA-seq]

| PRJNA751101 | ENA

PRC2 shields naive pluripotent cells from trophectoderm and mesoderm differentiation [ChIP-seq]

Project description:PRC2 shields naive pluripotent cells from trophectoderm and mesoderm differentiation [ChIP-seq]

| PRJNA751100 | ENA

PRC2 shields naive pluripotent cells from trophectoderm and mesoderm differentiation.

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-08-23 | GSE181244 | GEO

RNA-seq of H9 cell line during differentiation from naïve extraembryonic endoderm to extraembryonic mesoderm.

Project description:Extraembryonic mesoderm (ExM) is one of the first cell types that emerges during embryogenesis and constitutes essential supportive tissues for the pregnancy. Primate ExM is known to form prior to gastrulation, unlike its murine counterpart which is derived from the primitive streak. Based on the embryonic morphology and the proximity of ExM to the extraembryonic endoderm (hypoblast), we hypothesised that ExM can be derived in vitro from the naïve extraembryonic endoderm (nEnd) cell line. We applied a mesoderm differentiation protocol, which has been reported to induce ExM from mouse epiblast stem cells, on human nEnd and analysed the transcriptome on day 0, 1, 2, 8 and 15.

2023-06-29 | E-MTAB-12517 | biostudies-arrayexpress

Human mural trophectoderm

Project description:The trophoblast cell lineage is specified as early as the blastocyst stage, leading to the individualization of trophectoderm from pluripotent cells of the inner cell mass. We used a double in vitro transcription mRNA amplification technique and compared trophectoderm with pluripotent stem cells.

2011-07-15 | GSE28504 | GEO

Modeling human extraembryonic mesoderm cells using naive pluripotent stem cells [scRNA_seq_timecourse_naiveTSC]

Project description:time course scRNAseq of naïve to trophoblast stem cells and extraembryonic mesoderm conversion and D70 sorted extraembryonic mesoderm. Samples were collected at day 0, 1, 2, 4, 8, 13 and 18.

2022-08-01 | GSE204779 | GEO

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