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Transcriptome sequence of hTERT-HPNE and PANC-1 cells upon hydrogen peroxide treatment

ABSTRACT: Transcriptome sequence of hTERT-HPNE and PANC-1 cells upon hydrogen peroxide treatment

PROVIDER: PRJNA804173 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17915403_1.fastq.gz	Fastqsanger.gz
	SRR17915403_2.fastq.gz	Fastqsanger.gz
	SRR17915404_1.fastq.gz	Fastqsanger.gz
	SRR17915404_2.fastq.gz	Fastqsanger.gz
	SRR17915405_1.fastq.gz	Fastqsanger.gz

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Transcriptome sequence of hTERT-HPNE and PANC-1 cells upon hydrogen peroxide treatment

Project description:To explore the whole transcriptional regulation of lncRNA and mRNA expression in hydrogen peroxide (H2O2) signaling, we used human pancreatic normal epithelial cell line hTERT-HPNE and pancreatic cancer cell line PANC-1 for H2O2 treatment and utilized transcriptome sequence to analyze the change of lncRNA and mRNA transcripts. Then we predicted the potential transcription factor for these significantly changed transcripts.

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Funil Reservoir and hydrogen peroxide

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Hydrogen peroxide stress and Zat12 over-expression in Arabidopsis.

Project description:In this experiment we tested the transcriptome of transgenic Arabidopsis seedlings (5-day-old) constitutively expressing the zinc-finger protein Zat12 (At5g59820) under the control of the 35S-CaMV promoter (Zat12). The transcriptome of these seedlings was compared to that of wild type seedlings grown under the same conditions (WT) and to that of wild type seedlings grown under the same conditions and subjected to a hydrogen peroxide stress (WT+H2O2). Hydrogen peroxide treatment was performed by applying 20 mM hydrogen peroxide for 1 hour. In parallel to these experiments transgenic plants expressing Zat12 were subjected to a similar hydrogen peroxide stress (Zat12+H2O2). All treatments were performed with similar size and age seedlings grown in liquid culture (MS) and sampled at the same time as described by Davletova et al., 2005. Experimenter name = Ron Mittler Experimenter phone = 1-775-784-1384 Experimenter fax = 1-775-784-1650 Experimenter department = Dept. of Biochemistry Experimenter institute = University of Nevada Experimenter address = MS200 Experimenter address = Reno Experimenter address = Nevada Experimenter zip/postal_code = 89557 Experimenter country = USA Keywords: genetic_modification_design; stimulus_or_stress_design

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