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Transcription factor ZBTB7A-deficiency effect on U87MG cells in vitro

ABSTRACT: Transcription factor ZBTB7A-deficiency effect on U87MG cells in vitro

PROVIDER: PRJNA804312 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17930451_1.fastq.gz	Fastqsanger.gz
	SRR17930451_2.fastq.gz	Fastqsanger.gz
	SRR17930452_1.fastq.gz	Fastqsanger.gz
	SRR17930452_2.fastq.gz	Fastqsanger.gz
	SRR17930453_1.fastq.gz	Fastqsanger.gz

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Transcription factor ZBTB7A-deficiency effect on U87MG cells in vitro

Project description:Analysis of glioblastoma cell line U87MG depleted for ZBTB7A (Zinc Finger and BTB Domain Containing 7A, also known as FBI1, POKEMON or LRF). ZBTB7A is transcription factor that represses the transcription of a wide range of genes involved in cell proliferation and differentiation. Results provide insight into the role of ZBTB7A in glioblastoma.

2023-02-08 | GSE196320 | GEO

Transcriptome U87MG TMZ resistant

Project description:Differential transcriptome analysis between control cells (U87MG), TMZ-resistant cells with continuous TMZ treatment (U87MG R50) and TMZ-resistant cells with interrupted treatment (U87MG OFF R50).

2024-02-13 | GSE253458 | GEO

ZBTB7A ChIP-seq under different DHT level

Project description:Zinc finger and BTB domain containing transcription repressor ZBTB7A has been recently reported as a tumor suppressor who plays important functions to prevent the progression of prostate cancer. However, the chromatin activity of ZBTB7A in prostate cancer cells remain unclear. In order to identify the cistrome and transcriptome of ZBTB7A, we performed ZBTB7A ChIP-seq in VCaP cells and RNA-seq in VCaP cells transfected with siRNA targeting ZBTB7A or non-targeting control, respectively (cells were grown in full serum). By using combined ChIP-seq and RNA-seq analyses in VCaP cells, we have precisely mapped the ZBTB7A binding sites and identified a subset of genes that are directly repressed by ZBTB7A. To study the chromatin interaction of ZBTB7A with androgen receptor (AR), we also performed ChIP-seq of ZBTB7A in VCaP cells stimulated with 10 nM DHT for 4 hours versus vehicle control (cells were hormone-depleted prior to DHT stimulation). Our results show that ZBTB7A binding is increased by androgen at AR and ZBTB7A overlapping sites.

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RNA-seq with knockdown ZBTB7A and NTC control

2019-09-12 | GSE123090 | GEO

Trancriptome sequencing of BGC823 with or without ZBTB7A knockdown

Project description:Tumor budding (TB), clusters of less than 5 cells dissociated from the tumor mass, is reported to predict nodal involvement and recurrence in multiple human cancers including gastric carcinoma (GC). However, it is not clear how TB forms. In this study, we determined the expression pattern of GAGE12I, CTNND1, Kif26B, CREB1 and ZBTB7A in human GC samples by IHC attempting to find any TB-related markers. The results showed that TB could predict lymph node metastasis and is negatively associated with the overall survival of GC patients. The expression of ZBTB7A in the tumor margin (invasive front), rather than the other four markers, was much higher than that inside the tumor and was positively correlated with TB score. Moreover, patients with higher marginal ZBTB7A expression had poorer prognosis. ZBTB7A could enhance the migration and invasion capabilities of GC cells in vitro. Mechanically, ZBTB7A might activate EGFR-MAPK-ERK and PI3K-AKT-mTOR pathway, which resulted in epithelial-mesenchymal transition (EMT) to induce the formation of TB. Thus, we concluded that higher ZBTB7A expression in the tumor margin may contribute to the dissociation of tumor cells from the tumor mass to form TB by initiating EMT via EGFR-MEK-ERK and PI3K-AKT-mTOR pathway.

2020-12-31 | GSE147199 | GEO

Expression data from U87MG subclones

Project description:U87MG is a glioblastoma cell line that shows substantial heterogeneity despite long-term passaging. We used microarrays to identify variations in gene expression that are associated with phenotypic differences among subclones derived from U87MG.

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Differential gene expression analysis of U87MG EGFRvA cells vs. U87MG EGFR cells

Project description:EGFRvA is a novel and widely-expressed EGFR isoform, whose upregulation is positively related to glioma grades. Intriguingly, it is the upregulation of EGFRvA but not EGFR that significantly correlates with a poor prognosis in glioma patients. Cancer cells expressing EGFRvA (relative to EGFR) display a higher invasive capacity and a lower sensitivity to EGFR tyrosine kinase inhibitors (TKIs). To investigate the significant differently expressed genes between U87MG EGFRvA cells and U87MG EGFR cells，microarray experiments were conducted.

2012-09-30 | GSE41230 | GEO

ZBTB7A prevents RUNX1-RUNX1T1 dependent clonal expansion of human hematopoietic progenitor cells

Project description:ZBTB7A is frequently mutated in acute myeloid leukemia (AML) with t(8;21) translocation. However, the oncogenic collaboration between mutated ZBTB7A and the RUNX1-RUNX1T1 fusion gene in AML t(8;21) remains unclear. Here, we investigate the role of ZBTB7A and its mutations in the context of normal and malignant hematopoiesis.

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Transcriptome U87MG TMZ resistant

Project description:Transcriptome U87MG TMZ resistant

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Project description:After performing an in-vivo screening with U87 glioblastoma cells transduced with a knockdown library several genes could be identified. LAPTM5 which was one of the candidates was further evaluated. Single knockdown of LAPTM5 in U87MG conferred a pro-invasive phenotype in-vitro and in-vivo. To decipher the underlying pathways U87MG control cells (U87RNAi) and U87shLAPTM5 were analyzed after in-vitro culture by a transcription profiling Array.

2017-12-07 | E-MTAB-6316 | biostudies-arrayexpress

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