Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Severe acute respiratory syndrome coronavirus 2

ABSTRACT: Genomic Surveillance of SARS-CoV-2 in South Africa

PROVIDER: PRJNA805055 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR21187700_1.fastq.gz	Fastqsanger.gz
	SRR21187700_2.fastq.gz	Fastqsanger.gz
	SRR21187701_1.fastq.gz	Fastqsanger.gz
	SRR21187701_2.fastq.gz	Fastqsanger.gz
	SRR21187702_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 1590

Similar Datasets

Severe acute respiratory syndrome coronavirus envelope protein regulates cell stress responses and apoptosis

Project description:Severe acute respiratory syndrome virus (SARS-CoV) that lacks the envelope (E) gene (rSARS-CoV-ΔE) is attenuated in vivo [1,2]. To identify factors that contribute to rSARS-CoV-ΔE attenuation, gene expression in cells infected by SARS-CoV with or without E gene was compared. Twenty-five stress response genes were preferentially upregulated during infection in the absence of the E gene. In addition, genes involved in signal transduction, transcription, cell metabolism, immunoregulation, inflammation, apoptosis and cell cycle and differentiation were differentially regulated in cells infected with rSARS-CoV with or without the E gene. Administration of E protein in trans reduced the stress response in cells infected with rSARS-CoV-ΔE, with respiratory syncytial virus, or treated with drugs, such as tunicamycin and thapsigarcin that elicit cell stress by different mechanisms. In addition, SARS-CoV E protein down-regulated the signaling pathway inositol-requiring enzyme 1 (IRE-1) of the unfolded protein response, but not the PKR-like ER kinase (PERK) or activating transcription factor 6 (ATF-6) pathways, and reduced cell apoptosis. Overall, the activation of the IRE-1 pathway was not able to restore cell homeostasis, and apoptosis was induced probably as a meassure to protect the host by limiting virus production and dissemination. The expression of proinflammatory cytokines was reduced in rSARS-CoV-ΔE-infected cells compared to rSARS-CoV-infected cells, suggesting that the increase in stress responses and the reduction of inflammation in the absence of the E gene contributed to the attenuation of rSARS-CoV-ΔE. We used Affymetrix microarrays (Human Genome U133 plus 2.0) to compare global gene expression between SARS-CoV-infected, mock-infected and SARS-CoV-ΔE-infected cells. For ech type of sample three hybridizations were carried-out (independent biological replicates).

2012-06-20 | E-GEOD-30589 | biostudies-arrayexpress

Severe acute respiratory syndrome coronavirus 2

Project description:SARS-CoV-2 Surveillance Sequencing

| PRJNA713804 | ENA

Severe acute respiratory syndrome coronavirus 2

Project description:SARS-CoV-2 Baseline Surveillance Umbrella Project

| PRJNA1113573 | ENA

Severe acute respiratory syndrome coronavirus 2

Project description:Puerto Rico SARS-CoV-2 Genomic Surveillance

| PRJNA870735 | ENA

Severe acute respiratory syndrome coronavirus 2

Project description:SARS-CoV-2 genomic surveillance at CUIMC-NYPH