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Distinct gene expression dynamics in developing and regenerating crustacean limbs

ABSTRACT: Distinct gene expression dynamics in developing and regenerating crustacean limbs

PROVIDER: PRJNA805179 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17973000.fastq.gz	Fastqsanger.gz
	SRR17973100.fastq.gz	Fastqsanger.gz
	SRR17973200.fastq.gz	Fastqsanger.gz
	SRR17973300.fastq.gz	Fastqsanger.gz
	SRR17973400.fastq.gz	Fastqsanger.gz

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Distinct gene expression dynamics in developing and regenerating crustacean limbs

Project description:Regenerating animals have the ability to reproduce body parts that were originally made in the embryo and subsequently lost due to injury. Understanding whether the process of regeneration mirrors development is an open question in most regenerative species. Here we take a transcriptomics approach to examine to what extent leg regeneration shows the same temporal patterns of gene expression as leg development in the embryo, in the crustacean Parhyale hawaiensis. We find that leg development in the embryo shows stereotypic temporal patterns of gene expression. In contrast, global patterns of gene expression during leg regeneration show a high degree of variation, related to the physiology of individual animals. A major driver of this variation is the molting cycle. After dissecting the transcriptional signals of individual physiology from regeneration, we obtain temporal signals that mark distinct phases of leg regeneration. Comparing the transcriptional dynamics of development and regeneration we find that, although both processes use largely the same genes, the temporal patterns in which these gene sets are deployed are different and cannot be systematically aligned.

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