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Genome-wide map of TRIM33 binding sites in primary dendritic cells

ABSTRACT: Genome-wide map of TRIM33 binding sites in primary dendritic cells

PROVIDER: PRJNA805539 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17983564_1.fastq.gz	Fastqsanger.gz
	SRR17983564_2.fastq.gz	Fastqsanger.gz
	SRR17983565_1.fastq.gz	Fastqsanger.gz
	SRR17983565_2.fastq.gz	Fastqsanger.gz
	SRR17983566_1.fastq.gz	Fastqsanger.gz

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Genome-wide map of TRIM33 binding sites in primary dendritic cells

Project description:The homeostatic control of dendritic cells (DCs) is critical for appropriate immune responses, yet the regulatory mechanisms are not fully elucidated. Genome-wide mapping of TRIM33 binding sites in primary DCs revealed a role of this molecule in DC transcription regulation, and therefore homeostasis.

2024-06-01 | GSE196586 | GEO

Genome-wide map of TRIM33 binding sites in the dendritic cell line Mutu DC1940

Project description:Transcriptional control of dendritic cell (DC) homeostasis has not been fully understood. Genome-wide binding analysis of TRIM33 in dendritic cells revealed a critical role in gene regulation for DC differentiation.

2024-06-01 | GSE193918 | GEO

Genome-wide map of TRIM33 binding sites in the dendritic cell line Mutu DC1940

Project description:Genome-wide map of TRIM33 binding sites in the dendritic cell line Mutu DC1940

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Genome-wide map of TRIM33, CDK9 and S2 Pol II binding sites in common dendritic cell progenitors

Project description:Transcriptional control of dendritic cell (DC) development has not been fully understood. TRIM33, a transcription co-factor was found to be crucial for transcription regulation during the development of DCs. Genome wide binding site analysis with CUT&Tag revealed co-localization of TRIM33 with CDK9 and Serine 2 phosphorylated RNA polymerase (S2 Pol II) in the common dendritic cell progenitors (CDPs).

2024-06-01 | GSE255136 | GEO

Genome-wide map of TRIM33, CDK9 and S2 Pol II binding sites in common dendritic cell progenitors

Project description:Genome-wide map of TRIM33, CDK9 and S2 Pol II binding sites in common dendritic cell progenitors

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RNA-seq profiling of wild type and Trim33-/- splenic dendritic cells

Project description:The homeostatic control mechanism of dendritic cells (DCs), including pDCs, cDC1s and cDC2s, is not fully elucidated. Transcriptome profiling of wildtype and TRIM33 conditional knockout mice revealed a key role of TRIM33 in maintaining the homeostasis of all DC subsets.

2024-06-01 | GSE193183 | GEO

Dynamics of TRIM33 binding in macrophages and effects of TRIM33 deletion on chromatin state during activation of primary macrophages.

Project description:To get insight into TRIM33 functions, TRIM33 ChIP-seq was carried out in murine macrophage cell line (RAW) and in bone marrow-derived macrophages (BMDM). The results showed that, in addition to its role in hematopoietic differentiation, TRIM33 may modulate PU.1 transcriptional activity during macrophage development and/or activation.To characterize the role of TRIM33 in macrophages, we bred TRIM33fl/fl mice with Lyz-Cre mice where the Cre recombinase gene is under the regulatory sequences of the Lyz gene that is expressed only in mature myeloid cells. Bone marrow cells from LyzCre/Trim33+/+ mice and LyzCre/Trim33flox/flox mice were differentiated in macrophages and treated during 0h, 4h, 12h and 24h with LPS. Using ChIP-seq, we provide a link between TRIM33 binding and H3K4me3 spreading on inflammatory genes in macrophages. Chromatin immunoprecipitations of TRIM33 and H3K4Me3 followed by multiparallel sequencing performed in murine bone marrow-derived macrophages (BMDM).

2015-11-23 | E-GEOD-43654 | biostudies-arrayexpress

RNA-seq profiling of wild type and Trim33-/- dendritic cell-related progenitors

Project description:The development of dendritic cells (DCs) is regulated by complex transcriptional networks. DCs originate from the multipotent progenitors (MPPs) in the bone marrow, which could further give rise to common lymphoid progenitors (CLPs) and common dendritic cell progenitors (CDPs). Whereas CDPs, which could be further divided into CD115+ and CD115- populations, give rise to both conventional (cDC) and plasmacytoid DCs (pDCs), CLP is an addtional source of pDCs. Transcriptome profiling of wild type and Trim33-/- MPPs, CD115- CDPs, and CLPs revealed a critical role of TRIM33 in the development of DCs.

2024-06-01 | GSE194246 | GEO

Dynamics of TRIM33 binding in macrophages and effects of TRIM33 deletion on chromatin state during activation of primary macrophages.

2015-11-23 | GSE43654 | GEO

Genome-wide screening identifies Trim33 as an essential regulator of dendritic cell differentiation [Trim33 RNA-Seq]

Project description:The development of all dendritic cells (DCs) including antigen-presenting conventional DCs (cDCs) and cytokine-producing plasmacytoid DCs (pDCs) is controlled by the growth factor Flt3 ligand (Flt3L) and its receptor Flt3. We genetically dissected this common Flt3L-driven pathway of DCs differentiation using CRISPR/Cas9-based dropout screening in a Flt3L dependent progenitor cell line. Genome-wide screening identified multiple regulators of DC differentiation including the glycosylphosphatidylinositol transamidase complex and arginine methyltransferase Carm1, whose role was confirmed in vivo. It also showed that negative regulators of mTOR signaling, including the subunits of TSC and GATOR1 complexes, restricted progenitor growth but enabled DC differentiation, demonstrating that a TSC- and GATOR1-mediated shutdown of mTOR is required for the process. An orthogonal screen focused on transcriptional regulators showed that Trim33 (TIF-1g) is required for DC differentiation. Conditional targeting in vivo revealed an essential role of Trim33 in the development of all DCs, but not of monocytes or granulocytes. In particular, inducible deletion of Trim33 caused a rapid loss of all DC progenitors, pDCs and the cross-presenting cDC1 subset. The loss of Trim33 from DC progenitors caused spontaneous interferon and inflammatory response, aberrant induction of macrophage-specific genes, and failure to induce DC differentiation program. Collectively, these data elucidate common mechanisms that control Flt3L-driven differentiation of the entire DC lineage, and identify Trim33 as its essential regulator.

2024-04-12 | GSE259392 | GEO

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