Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

TrmB-like family transcription factor OxsR regulates the oxidative stress response in Haloferax volcanii

ABSTRACT: TrmB-like family transcription factor OxsR regulates the oxidative stress response in Haloferax volcanii

PROVIDER: PRJNA807670 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR18052700_1.fastq.gz	Fastqsanger.gz
	SRR18052700_2.fastq.gz	Fastqsanger.gz
	SRR18052701_1.fastq.gz	Fastqsanger.gz
	SRR18052701_2.fastq.gz	Fastqsanger.gz
	SRR18052702_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 48

Similar Datasets

Transciptome profile of TrmB and TbsP in Haloferax volcanii [RNA-seq]

Project description:The hypersaline-adapted archaeon Haloferax volcanii exhibits remarkable plasticity in its morphology, biofilm formation, and motility in response to variations in nutrients and cell density. The transcriptional regulator TrmB maintains the rod shape in the related species Halobacterium salinarum by activating the expression genes involved in gluconeogenesis. Here we investigated the role of TrmB in Hfx. volcanii. The ∆trmB strain rapidly accumulated suppressor mutations in a gene encoding a novel transcriptional regulator, which we name TrmB suppressor, or TbsP. TbsP is required for adhesion to abiotic surfaces and maintains wild-type cell morphology and motility. To better understand the roles of TrmB and TbsP, we conducted expression profiling in ∆trmB, ∆tbsP, ∆trmB∆tbsP in response to glucose availability. TrmB and TbsP jointly regulate the transcription of gapII, fbp, and ppsA.

2023-12-01 | GSE248564 | GEO

TrmB-like family transcription factor OxsR regulates the oxidative stress response in Haloferax volcanii

Project description:Haloarchaea tolerate high levels of reactive species that are naturally present in hypersaline environments. Hundreds of sRNAs, transcripts, and proteins are found altered in abundance when haloarchaea are exposed to reactive species, yet the regulators that control these responses are not well characterized. Here we report the function of Haloferax volcanii OxsR (redox stress responsive regulator, HVO_2970), a standalone winged-helix DNA binding domain protein of the TrmB-like family. Here we demonstrate that OxsR is important for growth in the presence of hypochlorite and binds specific regions of genomic DNA during hypochlorite stress by ChIP-seq analysis. The OxsR-bound intergenic regions were located nearby operons encoding oxidative stress functions, including the biosynthesis of low molecular weight thiols and thiol relay systems. Further analysis by qRT-PCR revealed that OxsR functions during oxidative stress largely as a transcriptional activator, and occasionally as a repressor. The conserved cysteine residue (C24) of OxsR and a CG-rich binding motif upstream of BRE/TATA box promoter elements were found important in the transcriptional activation of select operons during hypochlorite stress. 3D-modeling of OxsR revealed C24 to be located at a homodimer interface formed by antiparallel α1 helices, suggesting that C24 forms an intersubunit disulfide bond in the presence of oxidant that stabilizes the homodimer. The phylogenetic distribution of OxsR homologs with the conserved cysteine suggests this type of redox signaling mechanism is widespread in Archaea.

2022-02-18 | GSE196894 | GEO

Genome-wide binding of TbsP (HVO_2861) in Haloferax volcanii [CHIP-seq]

Project description:The hypersaline-adapted archaeon Haloferax volcanii exhibits remarkable plasticity in its morphology, biofilm formation, and motility in response to variations in nutrients and cell density. The transcriptional regulator TrmB maintains the rod shape in the related species Halobacterium salinarum by activating the expression genes involved in gluconeogenesis. Here we investigated the role of TrmB in Hfx. volcanii. The ∆trmB strain rapidly accumulated suppressor mutations in a gene encoding a novel transcriptional regulator, which we name TrmB suppressor, or TbsP. TbsP is required for adhesion to abiotic surfaces and maintains wild-type cell morphology and motility. We identified TbsP binding sites in the presence and absence of glucose to better understand the role of TbsP. TbsP does not bind DNA in response to glucose availability. The sole binding site near a metabolic enzyme-coding gene is upstream of gapII.

2023-12-01 | GSE248565 | GEO

Transciptome profile of TrmB and TbsP in Haloferax volcanii [RNA-seq]

Project description:Transciptome profile of TrmB and TbsP in Haloferax volcanii [RNA-seq]

| PRJNA1044788 | ENA

Haloferax volcanii DS2

Project description:Haloferax volcanii Arabinose

| PRJNA953033 | ENA

Haloferax volcanii DS2

Project description:Haloferax volcanii IPTG

| PRJNA953035 | ENA

Haloferax volcanii DS2

Project description:Haloferax volcanii Maltose