Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

NF-kB subunits direct kinetically distinct transcriptional cascades in antigen receptor-activated B cells

ABSTRACT: NF-kB subunits direct kinetically distinct transcriptional cascades in antigen receptor-activated B cells

PROVIDER: PRJNA808426 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

NF-kB subunits direct kinetically distinct transcriptional cascades in antigen receptor-activated B cells [ATAC-seq]

Project description:The NF-kB family of transcription factors orchestrates signal-induced gene expression in a diversity of cell types. Cellular responses to NF-kB activation are regulated at the level of cell- and signal-specificity, as well as differential use of family members (subunit specificity). Here we used time-dependent multi-omics to investigate selective functions of Rel and RelA, two closely related NF-kB proteins, in primary B lymphocytes activated via the B cell receptor. Despite large numbers of shared binding sites genome wide, Rel and RelA directed kinetically distinct cascades of gene expression in activated B cells. Single-cell RNA-Seq revealed marked heterogeneity of Rel- and RelA-specific responses and sequential binding of these factors was not a major mechanism of protracted transcription. Moreover, nuclear co-expression of Rel and RelA led to functional antagonism between the two factors. By rigorously identifying target genes of each NF-kB subunit, these studies provide insights into exclusive functions of Rel and RelA in immunity and cancer.

2023-05-25 | GSE197034 | GEO

NF-kB subunits direct kinetically distinct transcriptional cascades in antigen receptor-activated B cells [ChIP-seq]

2023-05-25 | GSE197032 | GEO

NF-kB subunits direct kinetically distinct transcriptional cascades in antigen receptor-activated B cells [scRNA-seq]

2023-05-25 | GSE197031 | GEO

NF-kB subunits direct kinetically distinct transcriptional cascades in antigen receptor-activated B cells [RNA-seq]

2023-05-25 | GSE197026 | GEO

NF-kB subunits direct kinetically distinct transcriptional cascades in antigen receptor-activated B cells [RNA-seq II]

Project description:The NF-kB family of transcription factors orchestrates signal-induced gene expression in a diversity of cell types. Cellular responses to NF-kB activation are regulated at the level of cell- and signal-specificity, as well as differential use of family members (subunit specificity). Here we used time-dependent multi-omics to investigate selective functions of Rel and RelA, two closely related NF-kB proteins, in primary B lymphocytes activated via the B cell receptor. Despite large numbers of shared binding sites genome wide, Rel and RelA directed kinetically distinct cascades of gene expression in activated B cells. Single cell RNA-Seq revealed marked heterogeneity of Rel- and RelA-specific responses and sequential binding of these factors was not a major mechanism of protracted transcription. Moreover, nuclear co-expression of Rel and RelA led to functional antagonism between the two factors. By rigorously identifying target genes of each NF-kB subunit, these studies provide insights into exclusive functions of Rel and RelA in immunity and cancer.

2023-05-25 | GSE225242 | GEO

NF-kB subunits direct kinetically distinct transcriptional cascades in antigen receptor-activated B cells [scRNA-seq]

Project description:NF-kB subunits direct kinetically distinct transcriptional cascades in antigen receptor-activated B cells [scRNA-seq]

| PRJNA808428 | ENA

NF-kB subunits direct kinetically distinct transcriptional cascades in antigen receptor-activated B cells [RNA-seq]

Project description:NF-kB subunits direct kinetically distinct transcriptional cascades in antigen receptor-activated B cells [RNA-seq]

| PRJNA808414 | ENA

NF-kB subunits direct kinetically distinct transcriptional cascades in antigen receptor-activated B cells [ChIP-seq]

Project description:NF-kB subunits direct kinetically distinct transcriptional cascades in antigen receptor-activated B cells [ChIP-seq]

| PRJNA808431 | ENA

NF-kB subunits direct kinetically distinct transcriptional cascades in antigen receptor-activated B cells [ATAC-seq]

Project description:NF-kB subunits direct kinetically distinct transcriptional cascades in antigen receptor-activated B cells [ATAC-seq]

| PRJNA808430 | ENA

NF-kB subunits direct kinetically distinct transcriptional cascades in antigen receptor-activated B cells [RNA-seq II]

Project description:NF-kB subunits direct kinetically distinct transcriptional cascades in antigen receptor-activated B cells [RNA-seq II]

| PRJNA934395 | ENA

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data