Ontology highlight
ABSTRACT:
PROVIDER: PRJNA808857 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| Other | ||||
| SRR18162100_1.fastq.gz | Fastqsanger.gz | |||
| SRR18162100_2.fastq.gz | Fastqsanger.gz | |||
| SRR18162200_1.fastq.gz | Fastqsanger.gz | |||
| SRR18162200_2.fastq.gz | Fastqsanger.gz |
Items per page: 5 1 - 5 of 1093 |
Similar Datasets
Project description:The goal of this study was to use global gene expression as a diagnostic tool to compare hepatic gene expression patterns in both male and female FHM in streams with the lowest and highest reproductive success, and potentially identify a suite of mRNA transcripts indicative of reproduction in a population The goal of this study was to compare differences in hepatic mRNA expression between gender at high and low egg-producing streams, not differences between individual streams. A k-means cluster analysis was performed using eggs/pair/day on the original 17 streams to delineate 3 clusters: high, medium and low. From that analysis, FHM from 6 of the original 17-streams used in Crago et al. (2010) were chosen for the microarray experiment (Fig. 1, Table 1). In this study the experimental condition is reproductive success; High versus Low reproductive success. The streams grouped into High Reproductive Success were Oak Creek-2007 (2313 eggs), Point Creek (1277 eggs), Meeme Creek (1164 eggs) and Baird Creek (967 eggs). The streams grouped into Low Reproductive Success were: Ashwaubenon Creek (0 eggs), Devils Creek (541 eggs) and Oak Creek-2006 (642 eggs). Multiple regression analysis using the 22 sediment and water quality characteristics measured in the 6 streams with the highest (n = 4 and lowest (n = 3) streams demonstrated that there were no differences amongst the streams in regards to measure sediment and water variables. .5 One array was run for each gender from each stream. So that Males from Point Creek were pooled and run on one array, males from Ashwaubenon Creek were run on a separate array, and so forth. There were 14 arrays used in this study, 7 for males, 7 for females from individual rivers. So that Males from Point Creek were pooled and run on one array, males from Ashwaubenon Creek were run on a separate array, and so forth. In the case of Oak Creek, which was sampled in both years, there was a large difference in egg production between two years. Therefore separate arrays were run for Oak Creek 2006 and Oak Creek 2007. All streams chosen had overall survival rates of at least 80% through the 21-day sampling period, except Devils River. The survival rate for Devils River was at 100% until four days prior to the end of the experiment when six fish died or escaped.
2014-04-09 | E-GEOD-56604 | biostudies-arrayexpress
Project description:New Zealand glacier-fed stream sediments samples.
| PRJEB40567 | ENA
Project description:Glacier-fed stream biofilms and water Raw sequence reads
| PRJNA1086791 | ENA
Project description:C2C12 myoblasts were infected with a retrovirus expressing Pax7d or with an empty virus (puro) as a control. All of the samples originated from the same common pool of parental C2C12. This pool was split into six streams. A single prep of Pax7d-puro virus was split into three volumes and used to infect three of the streams. A single prep of puro-alone virus was similarly split in three and used to infect the remaining three streams. From the point of the infection forward each stream was maintained distinct from the others. Cells were infected and grown simultaneously under identical conditions. The puro samples may be reused as controls for future experiments, and therefore were immediately hybridized to the MOE430A and MOE430B chips in order to generate a complete dataset. The Pax7d samples, as a specific component of this experiment, were only hybridized to the MOE430A chip as a first-look into the system. Experiment Overall Design: this experiment include 2 samples and 9 replicates
2008-06-12 | E-GEOD-3224 | biostudies-arrayexpress
Project description:While many heterologous molecules can be produced at trace concentrations via microbial bioconversion processes, maximizing their titers, rates, and yields from lignin-derived carbon streams remains challenging. Strong growth coupling can not only increase titers and yields but also shift the production period from stationary phase to growth phase. These methods however require multi-gene edits for implementation which may be initially perceived as impractical. Here, we evaluated 4,114 potential solutions to pair growth on para-coumarate to indigoidine production and prototype two cutsets in Pseudomonas putida KT2440. The implemented design was enhanced using adaptive lab evolution and the resulting strains were characterized for emergent phenotypes. Using x-ray tomography we revealed increased cell density in this strain. Proteomics identified two upregulated peroxidases that mitigate increased reactive oxygen species formation. Nine additional stepwise modifications informed by model-guided and rational approaches realized a growth coupled strain that produced 7.3 g/L indigoidine at 77% yield in minimal salt media. These ensemble strategies provide a blueprint for producing target molecules at high product titers, rates, and yields.
2024-02-29 | PXD040697 | Pride