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Effect of DGCR8 heterozygosity on steady state miRNA levels in embryonic teratocarcinoma PA-1 cells

ABSTRACT: Effect of DGCR8 heterozygosity on steady state miRNA levels in embryonic teratocarcinoma PA-1 cells

PROVIDER: PRJNA810448 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR18142981.fastq.gz	Fastqsanger.gz
	SRR18142982.fastq.gz	Fastqsanger.gz
	SRR18142983.fastq.gz	Fastqsanger.gz
	SRR18142984.fastq.gz	Fastqsanger.gz
	SRR18142989.fastq.gz	Fastqsanger.gz

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Effect of DGCR8 heterozygosity on steady state RNA levels in embryonic teratocarcinoma PA-1 cells

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DGCR8 haploinsufficiency leads to primate-specific RNA dysregulation and pluripotency defects

Project description:The 22q11.2 deletion syndrome (22qDS) is caused by a microdeletion in one of the chromosomes 22, comprising around 40 genes and including DGCR8, an essential gene for miRNA biogenesis and transposable elements control. Most of the clinical manifestations of 22qDS have their origin during embryonic development, but the contribution of human DGCR8 hemizygosity to the disease is unknown. To clarify the overall effect of DGCR8 in the context of 22qDS, we have generated two human embryonic models of DGCR8 +/-.In this dataset we use small RNA-seq to study the effect of DGCR8 heterozygosity on steady state miRNA levels in human embryonic stem cells.

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