Project description:Chromosome-level genome construction of Japanese three-spined stickleback using ultra-dense linkage analysis using single-cell sequencing of sperms
Project description:Symplocarpus nipponicus, a member of the Araceae family, is an endangered plant in several prefectures in Japan. For the conservation of this wild species, we investigated the morphology, life cycle, and genetic diversity of three wild populations. By fixed-point observation over several years, we found that it takes at least four years for the plant to set the inflorescences consisting of spadices and spathes, and another two years for it to set mature seeds. To examine the genetic diversity in the wild population, we developed 11 novel microsatellite markers and investigated the genetic variation in three populations in Kyoto Prefecture: Ayabe, Hanase, and Momoi. The Ayabe population carried less genetic variation than the other two areas, suggesting the isolation of the habitat and thus a higher risk of extinction. Our results provide basic knowledge of the ecological aspects of S. nipponicus, as well as molecular techniques for the assessment of its genetic diversity, and thus are useful for the conservation of this endangered species.
Project description:The mitochondrial genome of a crab symbiotic with sea urchins, Echinoecus nipponicus, was completely sequenced for the first time. The total mitogenome length of E. nipponicus was 16,173 bp including 13 protein-coding genes, 2 rRNA genes, and 22 tRNA genes. The phylogenetic tree confirmed that E. nipponicus belonged to the subsection Heterotremata within Brachyura. This is the first record of the complete mitogenome for the subfamily Eumedoninae.