Project description:The whole regulon of the LTTR All3953 was determined at 3 h after Ci deficiency in the cyanobacterium Anabaena sp. PCC 7120 by ChIP-Seq analysis. A TAP-tagged version of the protein was used for the chromatin immunoprecipitation. A total of 142 peaks were found, mainly located in the chromosome of Anabaena.
Project description:In order to examine the response of Anabaena sp. PCC 7120 to an infection of the Cyanophage A1, we have used customized microarrays to identify genes potentially up- or downregulated by the infection. For this we compared cell culture before and after infection.
Project description:To examine Alr3614 influence on the expression of Anabaena sp. PCC 7120, we have used customized microarrays to identify genes potentially regulated by this short DNA-binding protein. We compared the expression of an Alr3614-deletion strain with an Alr3614-complementation strain.
Project description:The sustainability of crewed infrastructures on Mars will depend on their abilities to produce consumables on site. These abilities may be supported by diazotrophic, rock-leaching cyanobacteria: from resources naturally available on Mars, they could feed downstream biological processes and lead to the production of oxygen, food, fuels, structural materials, pharmaceuticals and more. The relevance of such a system will be dictated largely by the efficiency of regolith utilization by cyanobacteria. We therefore describe the growth dynamics of Anabaena sp. PCC 7938 as a function of MGS-1 concentration (a simulant of a widespread type of Martian regolith), of perchlorate concentration, and of their combination. To help devise improvement strategies and predict dynamics in regolith of differing composition, we identify the limiting element in MGS-1 - phosphorus - and its concentration-dependent effect on growth. Finally, we show that, while maintaining cyanobacteria and regolith in a single compartment can make the design of cultivation processes challenging, preventing direct physical contact between cells and grains may reduce growth. Overall, we hope for the knowledge gained here to support both the design of cultivation hardware and the modeling of cyanobacterium growth within.