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Loss of circSry leads to decreased γH2AX levels and impairs spermatogenesis

ABSTRACT: Loss of circSry leads to decreased γH2AX levels and impairs spermatogenesis

PROVIDER: PRJNA813151 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR18243696_1.fastq.gz	Fastqsanger.gz
	SRR18243696_2.fastq.gz	Fastqsanger.gz
	SRR18243697_1.fastq.gz	Fastqsanger.gz
	SRR18243697_2.fastq.gz	Fastqsanger.gz
	SRR18243698_1.fastq.gz	Fastqsanger.gz

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Loss of circSry leads to decreased γH2AX levels and impairs spermatogenesis

Project description:Purpose:To gain a deeper insight into how circSry regulates spermatogenesis in spermatocytes, RNA-sequencing (RNA-seq) was performed to analyze the genome-wide changes by circSry deletion.

2022-03-08 | GSE197971 | GEO

CircSry regulates spermatogenesis by enhancing γH2AX expression via sponging miR138-5p

Project description:CircSry regulates spermatogenesis by enhancing γH2AX expression via sponging miR138-5p

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Loss of circSRY reduces γH2AX level in germ cells and impairs mouse spermatogenesis.

Project description:Sry on the Y chromosome is the master switch of sex determination in mammals. It has been well established that Sry encodes a transcription factor that is transiently expressed in somatic cells of the male gonad, leading to the formation of testes. In the testis of adult mice, Sry is expressed as a circular RNA (circRNA) transcript. However, the physiological function of Sry circRNA (circSRY) remains unknown since its discovery in 1993. Here we show that circSRY is mainly expressed in the spermatocytes, but not in mature sperm or somatic cells of the testis. Loss of circSRY led to germ cell apoptosis and the reduction of sperm count in the epididymis. The level of γH2AX was decreased, and failure of XY body formation was noted in circSRY KO germ cells. Further study demonstrated that circSRY directly bound to miR-138-5p in spermatocytes, and in vitro assay suggested that circSRY regulates H2AX mRNA through sponging miR-138-5p. Our study demonstrates that, besides determining sex, Sry also plays an important role in spermatogenesis as a circRNA.

| S-EPMC9684031 | biostudies-literature

γH2AX-associated RNA in mouse spermatocytes

Project description:In the testis of the mice, most spermatogonia are in pachytene stage. We have harvested spermatogonia from 3-week-old mice. As γH2AX is the marker of the XY body, we performed RNA-seq following RNA-ChIP using anti-γH2AX antibody in germ cells to report the γH2AX associated RNA in mouse spermatocytes, while IgG as the negative control. Process of RNA-ChIP for germ cells was as the ChIP protocol with some modifications.

2021-09-26 | GSE184711 | GEO

CircSry regulates spermatogenesis by enhancing γH2AX expression via sponging miR138-5p

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Dysfunction of CEP128 impairs spermatogenesis but not ciliogenesis in humans and mice

Project description:The centrosome is a conserved eukaryotic organelle essential for reproductive process, and centrosomal proteins (CEP) are necessary for composition of the centrosome. However, few CEPs have been genetically linked to fertility, and the related molecular mechanisms remains mysterious. Here, we identified a new CEP, CEP128, which is functional in spermatogenesis, fertilization and embryonic development in both humans and mice. The variants of CEP128/Cep128 could lead to aberrant centrosome structures of the sperm inducing to anomalies in sperm morphology, count as well as motility, and further result in male infertility, but did not grossly affect ciliogenesis. Mechanistically, both loss and up-regulation of CEP128 could cause suppressed expressions of the genes involved in the spermatogenesis and fertilization phase. Altogether, our findings unprecedentedly unveil a crucial role of CEP128 in male fertility and provides new insight into the function of CEPs in human disease.

2022-05-19 | PXD022731 | Pride

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