Ontology highlight
ABSTRACT:
PROVIDER: PRJNA813333 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| Other | ||||
| JAOAMX01.dat.gz | Other | |||
| JAOAMX01.fasta.gz | Fasta.gz | |||
| JAOAMX01.master.dat | Other | |||
| JASEVX01.dat.gz | Other |
Items per page: 5 1 - 5 of 143 |
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Project description:This is a dataset generated by the Drosophila Regulatory Elements modENCODE Project led by Kevin P. White at the University of Chicago. It contains ChIP-chip data on Agilent 244K dual-color arrays for antibody: CBP/p300 at 12 different time-points of Drosophila development. Current Dataset: [GSM386269..GSM386277]: ChIP-chip of CBP in Drosophila embryos at 4-8 hours of development [GSM386279..GSM386287]: ChIP-chip of CBP in Drosophila embryos at 20-24 hours of development [GSM418309..GSM418317]: ChIP-chip of CBP in adult female Drosophila [GSM418318..GSM418326]: ChIP-chip of CBP in adult male Drosophila [GSM418335..GSM418343]: ChIP-chip of CBP in Drosophila embryos at 16-20 hours of development [GSM418454..GSM418462]: ChIP-chip of CBP in Drosophila L3 larvae [GSM418463..GSM418471]: ChIP-chip of CBP in Drosophila pupae [GSM442425..GSM442433]: ChIP-chip of CBP in Drosophila embryos at 0-4 hours of development [GSM443119..GSM443127]: ChIP-chip of CBP in Drosophila L1 larvae For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf
2009-03-28 | GSE15427 | GEO
Project description:This is a dataset generated by the Drosophila Regulatory Elements modENCODE Project led by Kevin P. White at the University of Chicago. It contains ChIP-chip data on Agilent 244K dual-color arrays for antibody: CBP/p300 at 12 different time-points of Drosophila development. Current Dataset: [GSM386269..GSM386277]: ChIP-chip of CBP in Drosophila embryos at 4-8 hours of development [GSM386279..GSM386287]: ChIP-chip of CBP in Drosophila embryos at 20-24 hours of development [GSM418309..GSM418317]: ChIP-chip of CBP in adult female Drosophila [GSM418318..GSM418326]: ChIP-chip of CBP in adult male Drosophila [GSM418335..GSM418343]: ChIP-chip of CBP in Drosophila embryos at 16-20 hours of development [GSM418454..GSM418462]: ChIP-chip of CBP in Drosophila L3 larvae [GSM418463..GSM418471]: ChIP-chip of CBP in Drosophila pupae [GSM442425..GSM442433]: ChIP-chip of CBP in Drosophila embryos at 0-4 hours of development [GSM443119..GSM443127]: ChIP-chip of CBP in Drosophila L1 larvae For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf For each combination of time-point and antibody, triplicate ChIP experiments have been performed and hybridized on Agilent 244K arrays. 3 arrays per genome have been used so that each time-point is a set of 9 tiling arrays.
2010-06-21 | E-GEOD-15427 | biostudies-arrayexpress
Project description:Genome-wide distribution of histone H3K18 and H3K27 acetyltransferases, Crebbp (CBP) and Ep300 (p300), is used to map enhancers and promoters, but whether these elements functionally require CBP/p300 remains largely uncertain. We investigated this relationship by comparing genomic CBP recruitment with gene expression in wild type and CBP/p300 double-knockout fibroblasts. ChIP-seq revealed nearby CBP recruitment for 20 percent of constitutively expressed genes, but surprisingly, three-quarters of these were unaffected or slightly activated by CBP/p300 deletion. Computationally defined enhancer-promoter-units (EPUs) having a CBP peak within two kilobases of the enhancer-like element provided better predictive value, with CBP/p300 deletion attenuating expression of 40 percent of such EPU assigned constitutively expressed genes. We next examined signaling-responsive (Hypoxia Inducible Factor) gene expression and CBP recruitment, and found that 97 percent of inducible genes were within 50 kilobases of an inducible CBP peak, and 70 percent of these required CBP/p300 for full inducible expression. Unexpectedly however, most inducible CBP peaks occurred near signal-nonresponsive genes. eight samples total; Two wild type and two CBP null primary mouse embryonic fibroblast (MEF) lines, each treated with 100uM 2,2-dipyridyl or ethanol vehicle for 2 hours
2014-09-29 | E-GEOD-54453 | biostudies-arrayexpress
Project description:CREB-binding protein (CBP, also known as nejire) is a transcriptional co-activator that is conserved in metazoans. We have generated CBP ChIP-seq data from Drosophila S2 cells and compared it to modENCODE data. This shows that CBP is bound at genomic sites with a wide range of functions. As expected, we find that CBP is bound at active promoters and enhancers. In addition, we find that the strongest CBP sites in the genome are found at Polycomb Response Elements embedded in histone H3 lysine 27 trimethylated (H3K27me3) chromatin, where they correlate with binding of the Pho repressive complex. Interestingly, we find that CBP also binds to most insulators in the genome. At a subset of these, CBP may regulate insulating activity, measured as the ability to prevent repressive H3K27 methylation from spreading into adjacent chromatin. ChIP seq in Drosophila S2 cells using two different antibodies against CBP (nejire), one raised in rabbit against amino acids 2540-3190 (CBP rb), and one raised in guinea-pig against amino acids 1-178 (CBP gp)
2015-11-06 | E-GEOD-64464 | biostudies-arrayexpress