Project description:CX3CR1pos monocytes are mobilized upon infection and undergo monocyte-to-macrophage transition in inflamed tissues. Using scRNA-seq of CD11c+ cells from bronchoalveolar lavage fluid (BALF) infected with IAV (PR/8, H1N1 ,we demonstrate that, during severe viral pneumonia, bone marrow-derived macrophages (BMDM) pass co-ordinated trajectories of pro-inflammatory-to-tissue-healing phenotypes, before differentiating into tissue-resident alveolar macrophages, that retain a long-term tissue-protective phenotype.
Project description:This study aimed to delineate molecular phenotypes of the lung microenvironment across idiopathic interestitial pneumonias, namely interstitial pneumonia with autoimmune features (IPAF)and idiopathic pulmonary fibrosis (IPF) through proteomic analysis of bronchoalveolar lavage fluid (BALF).
Project description:Sepsis-induced acute lung injury (ALI) is prevalent in septic patients and has a high mortality rate. Considering ALI’s close link to sepsis, we used a Pseudomonas aeruginosa (PA) pneumonia-induced sepsis mouse model to investigate alveolar microenvironment alterations and lung injury post-sepsis. Peptidyl arginine deiminase (PADI) 2 and PADI4 are highly expressed in immune cells, and play substantial roles in the immune response to sepsis, but their specific functions remain unclear.We employed single-cell RNA sequencing (scRNA-seq) technology to map immune cell populations in bronchoalveolar lavage fluid (BALF) cells from Wild type (WT) and Padi2 and Padi4 double knock-out (DKO)mice in PA pneumonia-induced sepsis and sham conditions.
Project description:In dogs, a species for which markers of cell populations are often limiting, we sought to evaluate in an unbiased way the heterogeneity of cell subpopulations in the bronchoalveolar lavage fluid of healthy dogs, by single-cell RNA-sequencing.
Project description:The study is intended to find if correlation exists between the abundance of bacterial gene for pyruvate ferredoxin oxidoreductase (PFOR) and short-chain fatty acids concentration in bronchoalveolar lavage fluid from patients with HIV-Associated Bacterial Pneumonia.