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Transcription activated by AP2-Z in early zygote plays an essential role in Plasmodium ookinete development

ABSTRACT: Transcription activated by AP2-Z in early zygote plays an essential role in Plasmodium ookinete development

PROVIDER: PRJNA813561 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR18258607.fastq.gz	Fastqsanger.gz
	SRR18258608.fastq.gz	Fastqsanger.gz
	SRR18258609.fastq.gz	Fastqsanger.gz
	SRR18258610.fastq.gz	Fastqsanger.gz
	SRR18258611.fastq.gz	Fastqsanger.gz

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Transcription activated by AP2-Z in early zygote plays an essential role in Plasmodium ookinete development [ChIP-seq]

Project description:To investigate the genome-wide binding sites of early zygote AP2-family transcription factor, AP2-Z in Plasmodium berghei, the chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq) analyses were performed. Parasites expressing GFP-fused AP2-Z (AP2-Z::GFP) were harvested at 6 hours post starting ookinete culture (hpoc) and subjected to ChIP-seq experiments using anti-GFP antibody. Binding sites of AP2-Z were determined from the sequence data.

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Transcription activated by AP2-Z in early zygote plays an essential role in Plasmodium ookinete development [RNA-seq]

Project description:Plasmodium berghei transcriptomes of wild-type and ap2-z knockout parasites [ap2-z(-)] were analyzed at 6 hours post starting ookinete culture (hpoc).

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Transcription activated by AP2-Z in early zygote plays an essential role in Plasmodium ookinete development [RNA-seq]

Project description:Transcription activated by AP2-Z in early zygote plays an essential role in Plasmodium ookinete development [RNA-seq]

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Transcription activated by AP2-Z in early zygote plays an essential role in Plasmodium ookinete development [ChIP-seq]

Project description:Transcription activated by AP2-Z in early zygote plays an essential role in Plasmodium ookinete development [ChIP-seq]

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Plasmodium berghei WT and ap2-o disruptant ookinete culture 12h

Project description:Plasmodium berghei WT and ap2-o disruptant ookinete culture 12h Plasmodium berghei WT and ap2-o disruptant ookinete culture 12h

2010-05-14 | E-GEOD-14376 | biostudies-arrayexpress

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Project description:Plasmodium berghei WT and ap2-o disruptant ookinete culture 12h

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Identification of AP2-O targets [microarray]

Project description:Stage-specific transcription is a fundamental biological process in the lifecycle of the Plasmodium parasite. Proteins containing the AP2 DNA-binding domain are responsible for stage-specific transcriptional regulation, and belong to the only known family of transcription factors in Plasmodium parasites. A comprehensive identification of their target genes will advance our understanding of the molecular basis of stage-specific parasite development. In this study, we have used ChIP-seq technology to identify the target genes of the ookinete-specific AP2 transcription factor AP2-O.

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Plasmodium transcription repressor AP2-O3 regulates sex-specific identity of gene expression in female gametocytes [ChIP-seq]

Project description:Male and female gametocytes are sexual precursor cells essential for transmission of malaria parasite in the mosquitoes. Differentiation of gametocytes to fertile gametes (gametogenesis) relies on the gender-specific transcriptome. However, how the parasites establish distinct repertoire of gene transcription in the male and female gametocytes remains largely unknown. Here, we report that an Apetala2 (AP2) family transcription factor (TF) AP2-O3 operates as a transcription repressor regulating female gametocyte transcriptome. AP2-O3 is specifically localized in the nucleus of the female gametocytes. AP2-O3-deficient parasites produce apparently normal female gametocytes, which fail to differentiate to fully fertile female gametes, leading to developmental arrest in fertilization and early development post-fertilization. AP2-O3 disruption causes massive up-regulation of transcriptionally dormant male genes and simultaneously down-regulation of highly transcribed female genes in female gametocytes. ChIP-seq and EMSA analysis establish AP2-O3 as a transcription repressor that targets a significant proportion of the upregulated male genes by recognizing an eight-base DNA motif in the promoters. In addition, the maternal AP2-O3 is removed after fertilization, which is required for the zygote to ookinete development. These results demonstrate that global transcriptional repression of male genes in the female gametocytes is required for safeguarding female-specific transcriptome and essential for the mosquito transmission of Plasmodium.

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Plasmodium transcription repressor AP2-O3 regulates sex-specific identity of gene expression in female gametocytes [RNA-seq]

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