Circulating exosomal microRNAs contribute to the antimigraine effects of acupuncture
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PROVIDER: PRJNA814313 | ENA |
REPOSITORIES: ENA
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Project description:We conducted this study to determine whether exosome regulation underlies the antimigraine mechanisms of acupuncture. By comparing serum samples from patients with migraine and healthy controls using high-throughput small RNA sequencing technology , we identified 705 exosomal microRNAs that are differentially expressed in patients with migraine, and this set of 705 microRNAs included five that are particularly well characterised (hsa-miR-369-5p, hsa-miR-1268b, hsa-miR-145-5p, hsa-miR-222-5p, and hsa-miR-4488). By comparing serum samples collected from patients with migraine before and after acupuncture treatment, we showed that acupuncture normalised the expression levels of those five well-characterised exosomal microRNAs.
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Project description:Circulating exosomal microRNAs are indicative of patients with biliary colic
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Project description:MicroRNAs (miRNAs) are intrinsic regulators in the various cellular processes, and their abnormalities are considered to be involved in the onset of human disorders, including cancer. Circulating miRNA is focused as new cancer biomarker however it is regarded that circulating RNA are released not only from tumor but also by various pathways. Recently, exosomes, small membrane vesicles, have been a major interest in cancer research field, because of their unique biological properties. Exosomes are secreted from various cells and the components (Lipids, mRNAs, miRNAs and proteins) reflect origin of the cells secreting them. Identification of exosomal miRNAs from cancer cells is expected to provide useful biomarkers of cancer. To identify specific exosomal miRNAs as candidate biomarkers for colorectal cancer, we compared exosomal miRNA profiles of 5 colon cancer cell lines with that of normal colon-derived epithelial cells, and isolated a subset of miRNAs as commonly-secreted miRNAs from colon cancer cells Endogenously expression of microRNAs were analyzed by Agilent Human miRNA V3 Microarray (G4470C) using total RNAs of human colon-derived FHC cells and human colon cancer cell lines (HCT116 cells and SW480 cells) at two independent experiments. Exosomal microRNAs were analyzed by microRNA microarray using total RNAs of exosomes from conditioned media of FHC cells, HCT116 cells, and SW480 cells at three independent experiments.As negative control of exosomal microRNAs in conditioned media, FBS-exosomal microRNAs were analyzed at four independent experiments. Exosomes were prepared by step-wise ultra-centrifugation methods. RNA was prepared by Trizol or Trizol-LS reagent (Invitrogen) and RNeasy mini spin column (Qiagen).
2014-04-01 | E-GEOD-39814 | biostudies-arrayexpress
Project description:MicroRNAs (miRNAs) are intrinsic regulators in the various cellular processes, and their abnormalities are considered to be involved in the onset of human disorders, including cancer. Circulating miRNA is focused as new cancer biomarker however it is regarded that circulating RNA are released not only from tumor but also by various pathways. Recently, exosomes, small membrane vesicles, have been a major interest in cancer research field, because of their unique biological properties. Exosomes are secreted from various cells and the components (Lipids, mRNAs, miRNAs and proteins) reflect origin of the cells secreting them. Identification of exosomal miRNAs from cancer cells is expected to provide useful biomarkers of cancer. To identify specific exosomal miRNAs as candidate biomarkers for colorectal cancer, we compared exosomal miRNA profiles of 5 colon cancer cell lines with that of normal colon-derived epithelial cells, and isolated a subset of miRNAs as commonly-secreted miRNAs from colon cancer cells Endogenously expression of microRNAs were analyzed by Agilent Human miRNA V3 Microarray (G4470C) using total RNA of three human colon cancer cell lines (HT-29 cells, SW48 cells, and RKO cells) at two independent experiments. Exosomal microRNAs were analyzed by microRNA microarray using total RNA of exosomes from conditioned media of three human colon cancer cell lines, HT-29 cells, SW48 cells, and RKO cells at three independent experiments. Exosomes were prepared by step-wise ultra-centrifugation methods. RNA was prepared by Trizol or Trizol-LS reagent (Invitrogen) and RNeasy mini spin column (Qiagen).
2014-04-01 | E-GEOD-39832 | biostudies-arrayexpress
Project description:Circulating exosomal microRNAs as predictive biomarkers of neoadjuvant chemotherapy response in breast cancer
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Project description:Differential expression of circulating exosomal microRNAs in refractory intracranial atherosclerosis associated with antiangiogenesis
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Project description:Background: Gallstone disease (GSD) is one of the most prevalent and costly digestive system diseases in the world and can lead to pancreatitis, severe biliary tract infection and malignant biliary tract tumors. Gallstones can induce biliary colic, and the pain radiates to the shoulder and back. Severe attacks cause nausea and vomiting, which seriously affect the patient's work and life. Exosomes are important extracellular vesicles, and exosomal microRNAs (miRNAs) can be used as biomarkers and therapeutic targets for a variety of diseases. To the best of our knowledge, exosomal miRNA has not been well evaluated as a biomarker of the progression of cholelithiasis or the onset of biliary colic. Methods: Plasma samples from 10 patients with biliary colic attack were collected for exosome extraction, identification and miRNA sequencing. The exosome miRNA sequencing data of 10 healthy people in the GEO database were used as controls for data analysis. Results: Comparison of the plasma exosomal miRNAs of patients with biliary colic to those of healthy controls showed that 35 exosomal miRNAs were upregulated and 9 miRNAs were downregulated. GO and KEGG analyses showed that differential miRNAs are involved in the inflammatory response. The analysis of the subject operation characteristic curve shows that the expression of plasma exosomal miR-1268a can better reflect the attack characteristics of biliary colic than other miRNAs. miR-146a-3p participates in body inflammation and lipid metabolism processes and can be used as a potential therapeutic target. Conclusion: Plasma exosomal miRNAs can be used as biomarkers during the onset of biliary colic and can also be used as potential molecular targets to inhibit inflammation and analgesia.
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Project description:To investigate the possibilities of circulating exosomal miRNAs in the early screening and prevention of diabetic retinopathy(DR), and to explore how the exosomal miRNAs functioning in DR. We then performed gene expression profiling analysis using data obtained from small RNA-seq of 3 diabetes mellitus(DM) patients and 3 DR patients.
2023-07-12 | GSE221535 | GEO
Project description:Purpose:We aimed to investigate the role of circulating exosomal microRNAs (e-miRNAs) in recurrent ischemic events in ICAD Methods: consecutive patients with severe ICAD undergoing intensive medical management (IMM) were prospectively enrolled. Those with recurrent ischemic events despite IMM during 6-month follow up were algorithmically matched to IMM responders. Baseline blood e-miRNA expression levels of the matched patients were measured using next generation sequencing Results: a total of 122 e-miRNAs were isolated from blood samples of 10 non-responders and 11 responders. Thirteen e-miRNAs predicted IMM failure with 90% sensitivity and 100% specificity. Ingenuity pathway analysis (IPA) determined 10 of the 13 e-miRNAs were significantly associated with angiogenesis-related biological functions (p < 0.025) and angiogenic factors that have been associated with recurrent ischemic events in ICAD. These e-miRNAs included miR-122-5p, miR-192-5p, miR-27b-3p, miR-16-5p, miR-486-5p, miR-30c-5p, miR-10b-5p, miR-10a-5p, miR-101-3p, and miR-24-3p. As predicted by IPA, the specific expression profiles of these 10 e-miRNAs in non-responders had a net result of inhibition of the angiogenesis-related functions and up expression of the antiangiogenic factors conclusion: This study revealed distinct expression profiles of circulating e-miRNAs in refractory ICAD, suggesting an antiangiogenic mechanism underlying IMM failure.
2021-06-21 | GSE178500 | GEO