Project description:The fungal toxin-encoding genes are highly upregulated in the vegetative mycelium upon challenge with the predator. Our recent studies in microfluidics have shown that latter induction is spatially restricted to parts of the vegetative mycelium that is in direct contact with the predator. In order to dissect the defensome of a multicellular fungus against a predator, here, we performed RNA - sequencing of mushroom Coprinopsis cinerea upon challenged with fungivorous nematode Aphelenchus avenae in Microfluidics device at three different time points. We analyzed hyphae that were collected from a microfluidics device where they have been in direct contact with or cultivated without A. avenae.
Project description:Two mushrooms' species, Hericium and Pleurotus were grown on a mushroom substrate mixed with different precentage of olive mill solid waste (OMSW).
Mushroom fruit body (FB), spent mushroom substrate (SMS) and mushroom substrate alone without mushroom mycelium (MS) were extracted by methanol and injected to LC-MS/MS (Experimental details included in the methods and protocol's part). computational metabolomic tools have been used to study the effect of the OMSW on the diversity of the mushrooms specialized metabolism.
Project description:RNA sequencing was used to identify genome wide transcriptional changes occurring in the Drosophila mushroom body in juvenile and mature adult flies expressing a mushroom body-specific RNAi knockdown of Bap60. The results of this analysis suggested a role for Bap60 in the regulation of neurodevelopmental genes during a critical time window of juvenile adult brain development.
Project description:Two mushrooms' species, Hericium and Pleurotus were grown on a mushroom substrate mixed with different percentage of olive mill solid waste (OMSW). Mushroom fruit body (FB) spent mushroom substrate (SMS) and mushroom substrate alone without mushroom mycelium (MS) were extracted by methanol and injected to LC-MS/MS, using electro spray ionization (ESI) in a negative ion mode. (Experimental details included in the methods and protocol's part). computational metabolomic tools have been used to study the effect of the OMSW on the diversity of the mushrooms specialized metabolism.
Project description:Two mushrooms' species, Hericium and Pleurotus were grown on a mushroom substrate mixed with different percentage of olive mill solid waste (OMSW).
Mushroom fruit body (FB) spent mushroom substrate (SMS) and mushroom substrate alone without mushroom mycelium (MS) were extracted by methanol and injected to LC-MS/MS, using electro spray ionization (ESI) in a positive ion mode. (Experimental details included in the methods and protocol's part). computational metabolomic tools have been used to study the effect of the OMSW on the diversity of the mushrooms specialized metabolism.
Project description:Coprinopsis cinerea exhibits synchronised meiosis in the gill tissue of the fungus, which is 75% meiotic. The mushroom develops from a dikaryon, which contains two separate nuclei. These nucleifuse in the basidia (karyogamy). After karyogamy, the nuclei enter an extended meiotic prophase, in which pahcytene occurs at 6 hours post karyogamy (K+6). The tetrads produced by the second meiotic division are present 12 hours after karyogamy (K+12). To examine a comprehensive timecourse of meiosis in this organism, we took samples over a 15-hour period, 3 hours apart: K-3, K, K+3, K+6, K+9, K+12. Keywords: time course
Project description:The white button mushroom Agaricus bisporus is the most widely produced edible fungus with a great economical value. Its commercial cultivation process is often performed on wheat straw and animal manure based compost that mainly contains lignocellulosic material as a source of carbon and nutrients for the mushroom production. As a large portion of compost carbohydrates are left unused in the current mushroom cultivation process, the aim of this work was to study wild-type A. bisporus strains for their potential to convert the components that are poorly utilized by the commercial strain A15. Growth profiling suggested different abilities for several A. bisporus strains to use plant biomass derived polysaccharides, as well as to transport and metabolize the corresponding monomeric sugars. Six wild-type isolates with diverse growth profiles were compared for mushroom production to A15 strain in semi-commercial cultivation conditions. Transcriptome and proteome analyses of the three most interesting wild-type strains and A15 indicated that the unrelated A. bisporus strains degrade and convert plant biomass polymers in a highly similar manner. This was also supported by the chemical content of the compost during the mushroom production process. Our study therefore reveals a highly conserved physiology for unrelated strains of this species during growth in compost.
Project description:Coprinopsis cinerea exhibits synchronised meiosis in the gill tissue of the fungus, which is 75% meiotic. The mushroom develops from a dikaryon, which contains two separate nuclei. These nucleifuse in the basidia (karyogamy). After karyogamy, the nuclei enter an extended meiotic prophase, in which pahcytene occurs at 6 hours post karyogamy (K+6). The tetrads produced by the second meiotic division are present 12 hours after karyogamy (K+12). To examine a comprehensive timecourse of meiosis in this organism, we took samples over a 15-hour period, 3 hours apart: K-3, K, K+3, K+6, K+9, K+12. Keywords: time course Four biological replicate samples of each timepoint were taken. Labelled cDNA was hybridized to arrays in a 2 channel reaction. The reference sample was a mixture of timepoint samples.
