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Profile in in vitro produced bovine morulae treated with MAT2A inhibitor

ABSTRACT: Profile in in vitro produced bovine morulae treated with MAT2A inhibitor

PROVIDER: PRJNA814505 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR18289341_1.fastq.gz	Fastqsanger.gz
	SRR18289341_2.fastq.gz	Fastqsanger.gz
	SRR18289342_1.fastq.gz	Fastqsanger.gz
	SRR18289342_2.fastq.gz	Fastqsanger.gz
	SRR18289343_1.fastq.gz	Fastqsanger.gz

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Transcriptomic profile in in vitro produced bovine morulae treated with MAT2A inhibitor

Project description:We obtained transcriptomic profiles in bovine morulae which had been in vitro treated with a methionine adenosyltransferase 2A (MAT2A) inhibitor from the 8-16 cell stage.

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Genome-wide H3K27me3 profile in in vitro produced bovine morula treated with MAT2A inhibitor

Project description:We obtained genome-wide profiles of histone H3 lysine 27 trimethylation (H3K27me3) in bovine morula which had been in vitro treated with a methionine adenosyltransferase 2A (MAT2A) inhibitor from the 8-16 cell stage. Sequencing center: NODAI Genome Research Center, Tokyo University of Agriculture

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Transcriptomic profile in in vitro produced bovine morulae treated with MAT2A inhibitor

Project description:Transcriptomic profile in in vitro produced bovine morulae treated with MAT2A inhibitor

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Genome-wide H3K27me3 profile in in vitro produced bovine morula treated with MAT2A inhibitor

Project description:Genome-wide H3K27me3 profile in in vitro produced bovine morula treated with MAT2A inhibitor

| PRJNA814513 | ENA

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Project description:MAT2A inhibitor is dosed to MTAP null cell lines, samples are collected for transcriptome profiling

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Transcriptome Analysis Of Bovine Expanded Blastocysts Produced In Vivo Vs In Vitro Sof Opu

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Genome-wide mapping of MAT2A-associsted regions in bovine blastocysts

Project description:Methionine adenosyltransferase (MAT), that catalyzes the synthesis of S-adenosylmethionine (SAM) from ATP and methionine, is involved in folate-mediated one-carbon metabolism (OCM) that is essential for preimplantation embryos in terms of both short-term periconceptional development and long-term phenotypic programming beyond the periconceptional period. SAM is the universal methyl donor for epigenetic methylation of DNA and histones. In the context of the long-term phenotypic programming by the modulation of OCM during periconceptional period, possible interactions between MAT2A and epigenetic status of specific genes during this period are of particular interest. MAT2A has been proposed to interact with many chromatin-related proteins and be recruited to their specific target genes to constitute gene-regulatory complexes. Thus, in the aim of addressing the possible interaction of MAT2A with specific genomic DNA regions in periconceptional period, we performed a ChIP-seq analysis using MAT2A antibody against bovine blastocysts.

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MAT2A inhibitor in cancer cell lines

Project description:MAT2A inhibitor in cancer cell lines

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Project description:Sexual dimorphism in mammals is mostly attributable to sex-related hormonal differences in fetal and adult tissues; however, this may not be the sole determinant. Though genetically-identical for autosomal chromosomes, male and female preimplantation embryos could display sex-specific transcriptional regulation which can only be attributted to the differences in sexual chromosome dosage. We used microarrays to analyze sex-related transcriptional differences at the blastocyst stage. Day 7 bovine in vitro produced bovine blastocysts produced with sorted semen from 3 different bulls. Pooled RNA from 60 blastocysts of one sex and produced with one bull was used per chip. Three replicates of each sex per bull. In total, 18 Bovine GeneChip (Affymetrix) were used (3 replicates X 3 bulls X 2 sexes).

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Project description:RNA-seq analysis of Huh7 cells after MAT2A inhibitor (FIDAS-5) or BET bromodomain inhibitor (JQ1) treatment. The analysis revealed that inhibition of MAT2A or BET factors suppresses the expression of genes regulating cell cycle progression and additional cellular programs.

2022-02-28 | GSE184065 | GEO

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