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Cebp1 and Cebpβ transcriptional axis controls eosinophilopoiesis in zebrafish [ChIP-seq]

ABSTRACT: Cebp1 and Cebpβ transcriptional axis controls eosinophilopoiesis in zebrafish [ChIP-seq]

PROVIDER: PRJNA814539 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR18289965_1.fastq.gz	Fastqsanger.gz
	SRR18289965_2.fastq.gz	Fastqsanger.gz
	SRR18289966_1.fastq.gz	Fastqsanger.gz
	SRR18289966_2.fastq.gz	Fastqsanger.gz
	SRR18289967_1.fastq.gz	Fastqsanger.gz

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Cebp1 and Cebpβ transcriptional axis controls eosinophilopoiesis in zebrafish

Project description:Cebp1 and Cebpβ transcriptional axis controls eosinophilopoiesis in zebrafish

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Cebp1 and Cebpβ transcriptional axis controls eosinophilopoiesis in zebrafish [bulkRNA-Seq]

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Cebp1 and Cebpβ transcriptional axis controls eosinophilopoiesis in zebrafish [ChIP-seq]

Project description:Eosinophils are well known to regulate host protection from parasites and have been reported to paticipate many other physiologic and pathologic processes. Understanding the role of eosinophils in these processes requires a better understanding of eosinophilopoiesis. Using a zebrafish model, we have identified an eosinophil lineage-specific marker, eslec. Using this marker we have established a Tg(eslec:eGFP) reporter line, which specifically labels zebrafish eosinophil lineage cells from early life through adulthood. Spatial-temporal analysis of eslec+ cells demonstrated organ distribution at the larval stage. By single-cell RNA-Seq of eslec+ cells, tissue-distributed eosinophils were found to have similar differentiation paths but different tissue-specific expression profiles. Genetic analysis demonstrated a Cebp1 and Cebpβ transcriptional axis that regulated eosinophilopoiesis, in which Cebp1 directly targeted cebpb to inhibit eosinophil differentiationthe commitment, differentiation, and maturation of the eosinophil lineage. In summary, this study characterized eosinophil development in multiple dimensions including spatial-temporal patterns, expression profiles, and genetic regulators. The results provide for a better understanding of eosinophilopoiesis.

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Cebp1 and Cebpβ transcriptional axis controls eosinophilopoiesis in zebrafish [scRNA-Seq]

Project description:Eosinophils are well known to regulate host protection from parasites and have been reported to paticipate many other physiologic and pathologic processes. Understanding the role of eosinophils in these processes requires a better understanding of eosinophilopoiesis. Using a zebrafish model, we have identified an eosinophil lineage-specific marker, eslec. Using this marker we have established a Tg(eslec:eGFP) reporter line, which specifically labels zebrafish eosinophil lineage cells from early life through adulthood. Spatial-temporal analysis of eslec+ cells demonstrated organ distribution at the larval stage. By single-cell RNA-Seq of eslec+ cells, tissue-distributed eosinophils were found to have similar differentiation paths but different tissue-specific expression profiles. Genetic analysis demonstrated a Cebp1 and Cebpβ transcriptional axis that regulated eosinophilopoiesis, in which Cebp1 directly targeted cebpb to inhibit eosinophil differentiationthe commitment and differentiation of the eosinophil lineage. In summary, this study characterized eosinophil development in multiple dimensions including spatial-temporal patterns, expression profiles, and genetic regulators. The results provide for a better understanding of eosinophilopoiesis.

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Cebp1 and Cebpβ transcriptional axis controls eosinophilopoiesis in zebrafish.

Project description:Eosinophils are a group of granulocytes well known for their capacity to protect the host from parasites and regulate immune function. Diverse biological roles for eosinophils have been increasingly identified, but the developmental pattern and regulation of the eosinophil lineage remain largely unknown. Herein, we utilize the zebrafish model to analyze eosinophilic cell differentiation, distribution, and regulation. By identifying eslec as an eosinophil lineage-specific marker, we establish a Tg(eslec:eGFP) reporter line, which specifically labeled cells of the eosinophil lineage from early life through adulthood. Spatial-temporal analysis of eslec+ cells demonstrates their organ distribution from larval stage to adulthood. By single-cell RNA-Seq analysis, we decipher the eosinophil lineage cells from lineage-committed progenitors to mature eosinophils. Through further genetic analysis, we demonstrate the role of Cebp1 in balancing neutrophil and eosinophil lineages, and a Cebp1-Cebpβ transcriptional axis that regulates the commitment and differentiation of the eosinophil lineage. Cross-species functional comparisons reveals that zebrafish Cebp1 is the functional orthologue of human C/EBPεP27 in suppressing eosinophilopoiesis. Our study characterizes eosinophil development in multiple dimensions including spatial-temporal patterns, expression profiles, and genetic regulators, providing for a better understanding of eosinophilopoiesis.

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